Laboratory of Proteoglycan Signaling and Therapeutics, Hokkaido University Graduate School of Life Science, Sapporo 001-0021, Japan.
Biochem Biophys Res Commun. 2011 Aug 19;412(1):136-42. doi: 10.1016/j.bbrc.2011.07.059. Epub 2011 Jul 22.
The structure and biological activities of a highly sulfated heparan sulfate (HS) extracted from shrimp (Penaeus brasiliensis) heads were characterized. Structurally the shrimp HS was more heterogenous than heparin, although it is still highly sulfated. The molecular mass of the shrimp HS preparation was determined to be 32.3 kDa by gel filtration HPLC. Analysis by surface plasmon resonance demonstrated that various growth/differentiation factors specifically bound to the shrimp HS with comparable affinity. Notably, the shrimp HS had a greater inhibitory effect against infections by dengue virus type 2 as well as Japanese encephalitis virus than heparin. Experiments on anticoagulant activity indicated that the shrimp HS exhibited significant anti-thrombin activity, but less than the commercial heparin. Hence, the HS preparation from shrimp heads, an industrial waste, is a prospective agent for a variety of clinical applications.
从虾（Penaeus brasiliensis）头中提取的高度硫酸化的肝素硫酸酯（HS）的结构和生物学活性。从结构上看，尽管虾 HS 的硫酸化程度仍然很高，但它比肝素更为异质。虾 HS 制剂的分子量通过凝胶过滤 HPLC 确定为 32.3 kDa。表面等离子体共振分析表明，各种生长/分化因子特异性地与虾 HS 结合，亲和力相当。值得注意的是，虾 HS 对登革热病毒 2 型和日本脑炎病毒的感染具有更强的抑制作用，比肝素更强。抗凝血活性实验表明，虾 HS 具有显著的抗凝血酶活性，但低于商业肝素。因此，来自虾头的 HS 制剂是一种有前途的各种临床应用的药物。
