产 NDM-1 肠杆菌科细菌体外抗生素协同作用评价。
In vitro evaluation of antibiotic synergy for NDM-1-producing Enterobacteriaceae.
机构信息
Service de Bactériologie-Virologie, INSERM U914 'Emerging Resistance to Antibiotics', Hôpital de Bicêtre, Assistance Publique-Hôpitaux de Paris, Faculté de Médecine et Université Paris-Sud, K.-Bicêtre, Paris, France.
出版信息
J Antimicrob Chemother. 2011 Oct;66(10):2295-7. doi: 10.1093/jac/dkr296. Epub 2011 Aug 1.
OBJECTIVES
To analyse the in vitro activity of colistin, fosfomycin and tigecycline alone or in combination against enterobacterial NDM-1 producers.
METHODS
MIC values of colistin, fosfomycin and tigecycline were determined for 28 NDM-1-producing enterobacterial isolates. In vitro synergy combination testing was performed for eight clinical isolates and one Escherichia coli transconjugant (six being susceptible to the three antibiotics) using microdilution and chequerboard techniques.
RESULTS
MICs of colistin, fosfomycin and tigecycline were determined, showing that one-third of NDM-1-producing isolates were resistant or intermediate to at least one of the three drugs. Nevertheless, in vitro synergistic activity was observed for colistin plus fosfomycin and colistin plus tigecycline in very rare cases.
CONCLUSIONS
Synergistic activity was observed for colistin and fosfomycin, and colistin and tigecycline in rare cases, most of the interactions being indifferent.
目的
分析多黏菌素、磷霉素和替加环素单独或联合应用对产 NDM-1 肠杆菌的体外活性。
方法
测定了 28 株产 NDM-1 肠杆菌分离株对多黏菌素、磷霉素和替加环素的 MIC 值。采用微量稀释和棋盘微量稀释法对 8 株临床分离株和 1 株大肠杆菌转导体（对三种抗生素均敏感）进行体外协同组合试验。
结果
确定了多黏菌素、磷霉素和替加环素的 MIC 值，结果表明，三分之一的产 NDM-1 分离株对这三种药物中的至少一种存在耐药或中介现象。然而，在极少数情况下观察到多黏菌素加磷霉素和多黏菌素加替加环素具有协同活性。
结论
多黏菌素和磷霉素以及多黏菌素和替加环素在极少数情况下表现出协同活性，大多数相互作用为无关。
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