Department of Vision Sciences, Glasgow-Caledonian University, Cowcaddens Road, Glasgow, Scotland.
Eye Contact Lens. 2011 Sep;37(5):274-81. doi: 10.1097/ICL.0b013e318227f8c1.
To review the reported effects of contact lens wear on the surface epithelial cells of the human conjunctiva as assessed by conjunctival impression cytology (CIC).
A literature search was undertaken to identify reports on the conjunctival health after contact lens wear, principally as assessed using CIC.
Of 26 reports identified, 22 examined the bulbar conjunctiva, and 2 examined the tarsal conjunctiva. Just 16 reports provided data from which mean squamous metaplasia grades could be calculated, with the overall grade being just 0.7 on a 0 to 3 scale. Only 13 of these studies provided unambiguous data on the duration of contact lens wear, and only an apparent trend was evident in that grades of squamous metaplasia increased over early years of lens wear. Such a trend was not statistically significant either up to 6 years of average lens wear (P>0.05) or over all studies (P>0.5). The estimated variability in squamous metaplasia was substantially greater when low grades were reported, an observation that either reflects the heterogeneity in the cell response or highlights the difficulty in assigning low grades to cell samples.
Based on subjective grading, CIC studies reveal no clearly definable relationship between the duration of contact lens wear and the extent of development of conjunctival squamous metaplasia. Logically, therefore, objective methods to assess squamous metaplasia are needed. Various options for quantitative CIC are discussed, including the use of in vivo confocal microscopy.
通过结膜印迹细胞学（CIC）评估，综述软性隐形眼镜佩戴对人结膜表面上皮细胞的报道影响。
进行了文献检索，以确定使用 CIC 评估软性隐形眼镜佩戴后结膜健康状况的报告。
在确定的 26 份报告中，有 22 份检查了球结膜，2 份检查了睑结膜。仅有 16 份报告提供了可以计算鳞状上皮化生平均等级的数据，整体等级在 0 到 3 级的范围内仅为 0.7。其中仅 13 项研究提供了有关隐形眼镜佩戴持续时间的明确数据，而且仅在鳞状上皮化生等级随佩戴隐形眼镜的早期年限增加的情况下出现明显趋势。这种趋势在平均佩戴隐形眼镜 6 年内（P＞0.05）或在所有研究中（P＞0.5）均无统计学意义。当报告低等级时，鳞状上皮化生的可变性估计要大得多，这种观察结果要么反映了细胞反应的异质性，要么突出了将低等级分配给细胞样本的困难。
基于主观分级，CIC 研究表明，隐形眼镜佩戴时间与结膜鳞状上皮化生的发展程度之间没有明确的可定义关系。因此，从逻辑上讲，需要评估鳞状上皮化生的客观方法。讨论了各种定量 CIC 的选择，包括使用活体共聚焦显微镜。
