Silley P, Monsey D, Harris A M
Chemotherapy Department, Microbiology Division, Glaxo Group Research Limited, Greenford, UK.
J Antimicrob Chemother. 1990 Jan;25(1):83-90. doi: 10.1093/jac/25.1.83.
An in-vitro model was shown to be capable of simulating a cefuroxime serum profile equivalent to that observed in human volunteer studies, following a single dose of 250 mg cefuroxime axetil. The model was used to carry out kill kinetic studies and showed cefuroxime to lyse the four bacterial test strains, time of onset of lysis being related to the sensitivity of the respective organisms. The more sensitive Staphylococcus aureus and Haemophilus influenzae strains were subject to a higher absolute kill and showed no regrowth over the duration of the simulated serum profile. In contrast, Proteus mirabilis and Escherichia coli showed regrowth after 4 and 5 h respectively. The kill kinetic profiles of the respective organisms are discussed in relation to the pharmacokinetic analysis of the cefuroxime serum profile.
体外模型显示，单次服用250mg头孢呋辛酯后，该模型能够模拟出与人体志愿者研究中观察到的头孢呋辛血清谱相当的血清谱。该模型用于进行杀菌动力学研究，结果显示头孢呋辛可裂解四种受试细菌菌株，裂解开始时间与各菌株的敏感性相关。较敏感的金黄色葡萄球菌和流感嗜血杆菌菌株的绝对杀灭率更高，且在模拟血清谱持续时间内未出现再生长。相比之下，奇异变形杆菌和大肠杆菌分别在4小时和5小时后出现再生长。结合头孢呋辛血清谱的药代动力学分析，讨论了各菌株的杀菌动力学谱。
