University of Aberdeen, Rowett Institute of Nutrition & Health, Greenburn Road, Bucksburn, Aberdeen, UK.
Proteomics. 2011 Oct;11(19):3928-34. doi: 10.1002/pmic.201100163. Epub 2011 Sep 2.
Consumption of industrial trans fat raises the risk of cardiovascular disease, but it is unclear whether cis9,trans11-conjugated linoleic acid (CLA)--a trans fatty acid in dairy products--modulates disease development. We investigated the effects of complete diets providing 7% of energy as industrial trans fat or cis9, trans11 CLA, compared with oleic acid, on regulation of plasma proteins in 12 healthy men. Diets were provided for 3 wk each, in random order. Plasma was collected at the end of each 3 wk intervention period, depleted of its 12 most abundant proteins and analyzed by 2-DE. Principal component analysis of protein spot intensity values revealed that the nature of the dietary intervention did not significantly affect the plasma proteome. The intervention provided in the 1st period produced a significant treatment effect compared with the interventions provided in the other two periods, and there was a significant subject effect. In conclusion, the nature of an extreme dietary intervention, i.e. 7% of energy provided by industrial trans fat or cis9,trans11 CLA, did not markedly affect the plasma proteome. Thus plasma proteomics using 2-DE appears, by and large, an unsuitable approach to detect regulation of plasma proteins due to changes in the diet.
食用工业反式脂肪会增加心血管疾病的风险，但目前尚不清楚乳制品中的顺式 9，反式 11-共轭亚油酸（CLA）——一种反式脂肪酸——是否会调节疾病的发展。我们研究了提供 7%能量的全脂饮食作为工业反式脂肪或顺式 9，反式 11 CLA，与油酸相比，对 12 名健康男性血浆蛋白调节的影响。饮食以随机顺序提供 3 周。在每个 3 周干预期结束时收集血浆，从其 12 种最丰富的蛋白质中去除，并通过 2-DE 进行分析。蛋白质斑点强度值的主成分分析表明，饮食干预的性质并没有显著影响血浆蛋白质组。与其他两个时期提供的干预措施相比，第一期提供的干预措施产生了显著的治疗效果，并且存在显著的个体效应。总之，极端饮食干预的性质，即 7%的能量来自工业反式脂肪或顺式 9，反式 11 CLA，并没有显著影响血浆蛋白质组。因此，使用 2-DE 的血浆蛋白质组学似乎总体上是一种不适合检测由于饮食变化而导致的血浆蛋白质调节的方法。
