Zhang Huimin, Song Jian, Shi Junying
Shandong Academy of Traditional Chinese Medicine, Jinan 250014, China.
Zhongguo Zhong Yao Za Zhi. 2011 Apr;36(8):1071-4.
The candidate anti-influenza virus serum marker of Lonicera japonica was searched by comparing the serum protein discrepancy of experimental groups. It will provide the basis for setting forth the action and mechanism of anti-influenza virus.
Two-dimensional electrophoresis (2-DE) was used to separate different experimental groups and the differences in serum protein was compared. The significantly expressed protein spots was selected for mass spectrum identification and bioinformatics analysis.
Fifteen identified points of protein have more typical differences between experimental groups. The structures were identified by MALDI-TOF-MS, and 12 functional protein component ownership were retrieved.
There was significant difference between the serum protein of experimental groups Proteomic methods can be used to select and identify the serum marker. It is expected to clarify the mechanism of anti-influenza virus of L. japonica.
通过比较各实验组血清蛋白差异，探寻金银花抗流感病毒血清标志物，为阐明金银花抗流感病毒作用及机制提供依据。
采用双向电泳（2-DE）分离不同实验组，比较血清蛋白差异，选取差异显著的蛋白点进行质谱鉴定和生物信息学分析。
15个已鉴定的蛋白点在实验组间有较典型差异，通过基质辅助激光解析电离飞行时间质谱（MALDI-TOF-MS）鉴定其结构，检索到12种功能蛋白成分归属。
实验组血清蛋白存在显著差异，蛋白质组学方法可用于筛选和鉴定血清标志物，有望阐明金银花抗流感病毒机制。
