Hong H A, Loubser A S, de Assis Rosa D, Naranbhai V, Carr W, Paximadis M, Lewis D A, Tiemessen C T, Gray C M
AIDS Virus Research Unit, National Institute for Communicable Diseases, National Health Laboratory Services, Johannesburg, South Africa.
Tissue Antigens. 2011 Sep;78(3):185-94. doi: 10.1111/j.1399-0039.2011.01749.x.
The effector function of natural killer (NK) cells is modulated by surface expression of a range of killer-cell immunoglobulin-like receptors (KIRs) that interact with human leukocyte antigen (HLA) class I ligands. We describe the use of real-time polymerase chain reaction (PCR) assays that allow easy and quick detection of 16 KIR genes and the presence/absence of KIR-ligands based on allelic discrimination at codon 80 in the HLA-A/B Bw4 and HLA-C C1/C2 genes. These methods overcome the tedious and expensive nature of conventional KIR genotyping and HLA class I typing using sequence-specific primer (SSP) PCR, sequence-specific oligonucleotide (SSO) hybridization or sequence-based typing (SBT). Using these two cost-effective assays, we measured the frequencies of KIRs, KIR-ligands and KIR/KIR-ligand pairs in a cohort of Black women recruited in South Africa.
自然杀伤(NK)细胞的效应功能受到一系列杀伤细胞免疫球蛋白样受体(KIR)表面表达的调节,这些受体与人类白细胞抗原(HLA)I类配体相互作用。我们描述了实时聚合酶链反应(PCR)检测方法的应用,该方法基于HLA - A/B Bw4基因第80位密码子的等位基因鉴别以及HLA - C C1/C2基因,能够轻松快速地检测16个KIR基因以及KIR配体的有无。这些方法克服了使用序列特异性引物(SSP)PCR、序列特异性寡核苷酸(SSO)杂交或基于序列的分型(SBT)进行传统KIR基因分型和HLA I类分型的繁琐和昂贵的缺点。使用这两种经济高效的检测方法,我们测量了在南非招募的一组黑人女性中KIR、KIR配体和KIR/KIR配体对的频率。