Department of Anesthesiology, the 2nd Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Pain Med. 2011 Sep;12(9):1427-34. doi: 10.1111/j.1526-4637.2011.01193.x. Epub 2011 Aug 2.
Recently, evidence has accumulated to show that cation-chloride cotransporters (CCCs) participate in the modulation of pain transmission at the spinal cord level. To investigate whether CCC inhibitors might affect surgical pain, we examined the effect of furosemide in a rat incisional pain model.
We examined pain thresholds using von Frey filaments in intact and incision model Sprague-Dawley rats before and after intrathecal furosemide administration (100 µg/20 µL). Twenty-four rats were divided into four groups (N=6), groups A and B were intact rats, which received furosemide and its solvent, respectively. Groups C and D were incision rats, which received furosemide and its solvent, respectively. Non-parametric tests were used to calculate pain thresholds, and P<0.05 was considered significant.
Furosemide decreased the pain threshold (vs solvent) in intact rats for 2 hours after administration and caused excitatory behavior. However, furosemide increased the pain threshold in incision model rats ([1] at the incision point: at 20 minutes, 2 hours, 3 hours, 4 hours, and on the second to fifth days after incision; [2] at the remote point: at 20 minutes, 3-5 hours, and from the 3rd to the 7th day after incision) and improved wound recovery.
Intrathecal administration of the CCC inhibitor furosemide had antinociceptive effects in rats with incisional pain. Furosemide may be a novel treatment for postoperative pain.
最近有证据表明，阳离子-氯离子共转运体（CCCs）参与了脊髓水平疼痛传递的调节。为了研究 CCC 抑制剂是否会影响手术疼痛，我们在大鼠切口疼痛模型中研究了呋塞米的作用。
我们在鞘内给予呋塞米（100μg/20μL）前后，使用 von Frey 纤维检查完整和切口模型 Sprague-Dawley 大鼠的痛阈。24 只大鼠分为四组（每组 6 只），A 组和 B 组为完整大鼠，分别给予呋塞米和溶剂。C 组和 D 组为切口大鼠，分别给予呋塞米和溶剂。使用非参数检验计算痛阈，P<0.05 为差异有统计学意义。
呋塞米在给药后 2 小时降低了完整大鼠的痛阈（与溶剂相比），并引起兴奋行为。然而，呋塞米增加了切口模型大鼠的痛阈（[1]切口部位：在切口后 20 分钟、2 小时、3 小时、4 小时和第 2 至第 5 天；[2]在远隔部位：在 20 分钟、3-5 小时和切口后第 3 至第 7 天），并改善了伤口恢复。
鞘内给予 CCC 抑制剂呋塞米对切口痛大鼠具有镇痛作用。呋塞米可能是治疗术后疼痛的一种新方法。
