无复流现象扰乱了猪模型中连接蛋白 43 的表达和分布。

No-reflow disrupts the expression and distribution of connexin 43 in a swine model.

机构信息

Department of Cardiology, Fuwai Hospital and Cardiovascular Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100037, China.

出版信息

Microvasc Res. 2011 Nov;82(3):404-9. doi: 10.1016/j.mvr.2011.07.002. Epub 2011 Jul 21.

DOI:10.1016/j.mvr.2011.07.002

PMID:21810432

Abstract

INTRODUCTION AND OBJECTIVES

Ischemia and ischemia/reperfusion can dephosphorylate and redistribute connexin 43 (Cx43). But it is unknown whether no-reflow phenomenon has an effect on the expression and distribution of Cx43 after acute infarction and reperfusion.

METHODS

21 open-chest pigs were divided into three groups. Left anterior descending artery (LAD) occlusion for 90 min before 180 min of reperfusion was made in ischemia/reperfusion group. The pigs in ischemia groups were either subjected to LAD ligation for 90 min or for 270 min. No-reflow and risk regions were determined pathologically by dye staining. Cx43 expression was measured by western blotting and quantitative RT-PCR analysis. Cx43 spatial distribution was shown by immunofluorescence examination.

RESULTS

The content of phosphorylated and mRNA of Cx43 were higher in reflow region than in the no-reflow or sustained ischemic region. The distribution of Cx43 was also altered in no-reflow region.

CONCLUSIONS

There are some differences in synthesis, expression and distribution of myocardial Cx43 at microvascular level after ischemia/reperfusion. Cx43 is partially rephosphorylated with reperfusion only in the reflow myocardium.

摘要

介绍和目的

缺血和缺血/再灌注可使连接蛋白 43（Cx43）去磷酸化和重新分布。但尚不清楚无复流现象是否会对急性梗死和再灌注后 Cx43 的表达和分布产生影响。

方法

21 头开胸猪分为三组。在缺血/再灌注组中，左前降支（LAD）闭塞 90 分钟后再灌注 180 分钟。缺血组中的猪要么结扎 LAD 90 分钟，要么结扎 270 分钟。通过染色法病理确定无复流和危险区域。通过 Western blot 和定量 RT-PCR 分析测量 Cx43 的表达。通过免疫荧光检查显示 Cx43 的空间分布。