Department of Geriatric Medicine, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan.
Brain Res. 2011 Sep 2;1410:1-11. doi: 10.1016/j.brainres.2011.06.036. Epub 2011 Jun 23.
We investigated the differential immunoexpression of 14-3-3 proteins according to their 7 isoforms during the postnatal development of rat brains, primarily in the hippocampus, cortex, rostral migratory stream (RMS), olfactory bulb, and white matter. Wistar rats at different developmental stages, on postnatal days 2 (P2), P7, P14, P21 and P100 were obtained, and were incubated with each type of anti-14-3-3 isoform antibody. 14-3-3 common (COM)-like immunoreactivity (IR) which represents an epitope shared among the 7 isoforms was initially expressed in the olfactory bulb on P2. This IR was partially expressed in the dentate granule cells and hippocampal pyramidal neurons from P7, and increased during development. These chronological changes were similar to those obtained with beta, gamma, and eta isoforms. Epsilon isoform-like IR was initially identified in the cell body of cortical neurons and glia-like cells on P2. After P7, the IR was more intense in the neuropil of the cortex. This epsilon isoform-like IR was markedly accentuated in the stratum lucidum of the hippocampus after P7, where hippocampal mossy fibers terminate, functioning as a giant synapse. This suggests that epsilon isoforms may be associated with synaptogenesis of the hippocampal mossy fibers. Sigma isoform-like IR was observed in the nuclei of external plexiform layer cells of the olfactory bulb from P2 to P21, in the nuclei of the hippocampal pyramidal and dentate granule cells after P7 and in the nuclei of RMS cells after P7. Zeta and tau isoform-like IRs were mainly identified in the white matter and in oligodendroglial cells from P7 to P21. Different immunolocalizations of the 7 isoforms suggest that 14-3-3 protein isoforms are individually associated with neuronal development and synaptogenesis during postnatal formation of the rat brain.
我们研究了 14-3-3 蛋白在大鼠脑发育过程中的 7 种同工型的差异免疫表达，主要集中在海马体、皮层、嗅球和白质中。我们获得了不同发育阶段的 Wistar 大鼠，即出生后第 2 天（P2）、P7、P14、P21 和 P100，并使用每种类型的抗 14-3-3 同工型抗体进行孵育。在 P2 时，14-3-3 共同（COM）样免疫反应（IR）首先在嗅球中表达，该反应在 P7 时部分表达于齿状回颗粒细胞和海马锥体神经元，并在发育过程中增加。这些时间变化与 beta、gamma 和 eta 同工型的变化相似。epsilon 同工型样 IR 首先在 P2 时的皮质神经元和神经胶质样细胞的胞体中被识别。P7 后，IR 在皮层神经突中更为强烈。这种 epsilon 同工型样 IR 在 P7 后在海马的透明层中明显增强，海马苔藓纤维在此终止，作为巨大的突触。这表明 epsilon 同工型可能与海马苔藓纤维的突触发生有关。sigma 同工型样 IR 从 P2 到 P21 可见于嗅球外丛状层细胞的核中，从 P7 后可见于海马锥体和齿状回颗粒细胞的核中，从 P7 后可见于 RMS 细胞的核中。zeta 和 tau 同工型样 IR 主要在 P7 到 P21 期间在白质和少突胶质细胞中被识别。7 种同工型的不同免疫定位表明，在大鼠脑的出生后形成过程中，14-3-3 蛋白同工型分别与神经元发育和突触发生有关。
