Matin A, Cheng K L, Suen T C, Hung M C
Department of Tumor Biology, University of Texas, M.D. Anderson Cancer Center, Houston 77030.
Oncogene. 1990 Jan;5(1):111-6.
We studied the differential response of oncogene transformed NIH3T3 cells to glucocorticoids. As demonstrated for transformed human fibroblasts, the morphology of neu-, ras-, src- and sis-transformed mouse fibroblasts became more normal after glucocorticoid treatment. This change was not due to inhibition of the expression of oncogene mRNA or protein. However, the abl-transformed NIH3T3 cells were resistant to glucocorticoid-induced morphology change. These results indicate that the glucocorticoid-induced morphology change is specific to certain oncogene-transformed NIH3T3 cells. Transformed human fibroblasts generally have reduced amounts of cell surface fibronectin. When treated with glucocorticoids, they incorporate higher levels of fibronectin in their extracellular matrix, which correlates with their change in morphology. However, we found that, except for abl-transformed cells, the fibronectin level of the other oncogene transformed mouse cells was similar to non-transformed cells. Moreover, treatment of the neu-, ras-, src- and sis-transformed cells with glucocorticoids resulted in a change in morphology but no increase in cell surface fibronectin. These studies demonstrate that the glucocorticoid-induced morphological change of oncogene-transformed NIH3T3 cells is not due to enhanced expression of fibronectin. Therefore, other mechanisms are responsible for this glucocorticoid-induced phenotypic change of oncogene-transformed cells.
我们研究了癌基因转化的NIH3T3细胞对糖皮质激素的差异性反应。正如在转化的人成纤维细胞中所证明的那样，神经（neu）、ras、src和sis转化的小鼠成纤维细胞在糖皮质激素处理后形态变得更正常。这种变化并非由于癌基因mRNA或蛋白质表达的抑制。然而，abl转化的NIH3T3细胞对糖皮质激素诱导的形态变化具有抗性。这些结果表明，糖皮质激素诱导的形态变化对某些癌基因转化的NIH3T3细胞具有特异性。转化的人成纤维细胞通常细胞表面纤连蛋白含量减少。当用糖皮质激素处理时，它们在细胞外基质中掺入更高水平的纤连蛋白，这与它们的形态变化相关。然而，我们发现，除了abl转化的细胞外，其他癌基因转化的小鼠细胞的纤连蛋白水平与未转化的细胞相似。此外，用糖皮质激素处理神经、ras、src和sis转化的细胞导致形态变化，但细胞表面纤连蛋白没有增加。这些研究表明，糖皮质激素诱导的癌基因转化的NIH3T3细胞形态变化并非由于纤连蛋白表达增强。因此，其他机制导致了癌基因转化细胞的这种糖皮质激素诱导的表型变化。
