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2型腺病毒DNA聚合酶在感染重组杆状病毒的昆虫细胞中的表达。

Expression of adenovirus type 2 DNA polymerase in insect cells infected with a recombinant baculovirus.

作者信息

Watson C J, Hay R T

机构信息

Department of Biochemistry and Microbiology, University of St Andrews, Fife, UK.

出版信息

Nucleic Acids Res. 1990 Mar 11;18(5):1167-73. doi: 10.1093/nar/18.5.1167.

DOI:10.1093/nar/18.5.1167
PMID:2181402
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC330431/
Abstract

Sequences encoding adenovirus type 2 DNA polymerase were placed under control of the polyhedrin promoter and inserted into the baculovirus Autographa californica nuclear polyhedrosis virus by homologous recombination. Insect cells infected with the recombinant virus produced substantial amounts of the adenovirus type 2 DNA polymerase protein which was functional in both DNA polymerase and replication initiation reactions. Thus, the baculovirus expression system can provide active adenovirus type 2 DNA polymerase that is produced in quantities suitable for biochemical and structural analysis.

摘要

编码腺病毒2型DNA聚合酶的序列置于多角体蛋白启动子的控制之下，并通过同源重组插入到杆状病毒苜蓿银纹夜蛾核型多角体病毒中。用重组病毒感染昆虫细胞后，产生了大量腺病毒2型DNA聚合酶蛋白，该蛋白在DNA聚合酶和复制起始反应中均具有功能。因此，杆状病毒表达系统能够提供活性腺病毒2型DNA聚合酶，其产量适合于生化和结构分析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/595c/330431/ef8d3d52aa58/nar00189-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/595c/330431/a2357ef335d6/nar00189-0077-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/595c/330431/3a0956a91876/nar00189-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/595c/330431/ef8d3d52aa58/nar00189-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/595c/330431/a2357ef335d6/nar00189-0077-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/595c/330431/3a0956a91876/nar00189-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/595c/330431/ef8d3d52aa58/nar00189-0080-a.jpg

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本文引用的文献

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Formation of a covalent complex between the 80,000-dalton adenovirus terminal protein and 5'-dCMP in vitro.80,000道尔顿腺病毒末端蛋白与5'-脱氧胞苷酸在体外形成共价复合物。
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