Institute for Sustainable Agriculture (IAS-CSIC), Alameda del Obispo s/n, E-14004 Córdoba, Spain.
J Sci Food Agric. 2012 Jan 30;92(2):351-7. doi: 10.1002/jsfa.4585. Epub 2011 Aug 4.
Alpha-tocopherol is the predominant tocopherol form in sunflower seeds. Sunflower lines that accumulate increased levels of beta-, gamma- and delta-tocopherol in seeds as well as lines with reduced and increased total seed tocopherol content have been developed. The objective of this research was to evaluate whether the modified tocopherol levels are expressed in plant tissues other than seeds.
Lines with increased levels of beta-, gamma- and delta-tocopherol in seeds also possessed increased levels of these tocopherols in leaves, roots and pollen. Correlation coefficients for the proportion of individual tocopherols in different plant tissues were significantly positive in all cases, ranging from 0.68 to 0.97. A line with reduced tocopherol content in seeds also showed reduced content in roots and pollen.
Genetic modifications producing altered seed tocopherol profiles in sunflower are also expressed in leaves, roots and pollen. Reduced total seed tocopherol content is mainly expressed at the root and pollen level. The expression of tocopherol mutations in other plant tissues will enable further studies on the physiological role of tocopherols and could be of interest for early selection for these traits in breeding programmes.
α-生育酚是葵花籽中主要的生育酚形式。已经开发出了在种子中积累更高水平的β-、γ-和δ-生育酚以及总种子生育酚含量降低和增加的葵花品种。本研究的目的是评估这些改性生育酚水平是否在种子以外的植物组织中表达。
在种子中生育酚水平升高的品系在叶片、根和花粉中也具有更高水平的这些生育酚。在所有情况下，不同植物组织中单个生育酚比例的相关系数均为正，范围从 0.68 到 0.97。种子中生育酚含量降低的品系在根和花粉中也显示出降低的含量。
在向日葵中产生改变的种子生育酚谱的遗传修饰也在叶片、根和花粉中表达。总种子生育酚含量的降低主要在根部和花粉水平上表达。生育酚突变在其他植物组织中的表达将使人们能够进一步研究生育酚的生理作用，并可能对这些性状的早期选择在育种计划中具有重要意义。
