Forensic Toxicology Research Laboratory, Laurentian University, 935 Ramsey Lake Rd., Sudbury, Ontario P3E2C6, Canada.
J Anal Toxicol. 2010 Oct;34(8):510-5. doi: 10.1093/jat/34.8.510.
Skeletal tissues from a domestic pig exposed to amitriptyline, diazepam, and pentobarbital were analyzed to determine the relative distribution of these drugs in bone. Following drug exposure and euthanasia, remains were allowed to decompose outdoors to complete skeletonization between summer 2007 and fall 2009. Remains were recovered and separated according to bone type. Twelve different bone types were pulverized and sampled in triplicate. Each bone sample underwent methanolic extraction (96 h, 50 °C), followed by solid-phase extraction and gas chromatography-mass spectrometry in the selected ion monitoring mode. Mass-normalized assay responses underwent ANOVA with post-hoc testing, revealing bone type as a main effect for all three drugs, but not for the diazepam metabolite (nordiazepam). The assay response varied with respect to bone type by factors of 27, 39, and 20 for pentobarbital, diazepam, and amitriptyline, respectively. The relative distribution between bone type was qualitatively similar for the three administered drugs analyzed for, with the largest response obtained from rib for all three drugs. This is the first study, to the authors' knowledge, of the distribution of different drugs in various decomposed skeletal tissues in a controlled experiment using an animal model of comparable physiology to humans. These data have implications for the interpretive value of forensic drug measurements in skeletal tissues.
对暴露于阿米替林、安定和戊巴比妥的家猪的骨骼组织进行了分析，以确定这些药物在骨骼中的相对分布。药物暴露和安乐死后，遗骸被允许在户外分解，以便在 2007 年夏季到 2009 年秋季之间完成骨骼化。遗骸被回收并根据骨骼类型进行分离。12 种不同的骨骼类型被粉碎并进行了三次重复采样。对每个骨骼样本进行甲醇提取（96 小时，50°C），然后在选定离子监测模式下进行固相萃取和气相色谱-质谱分析。质量归一化分析物响应进行方差分析和事后检验，结果表明，所有三种药物的骨骼类型都是主要影响因素，但安定的代谢物（去甲安定）不是。对于戊巴比妥、安定和阿米替林，骨型的分析物响应分别变化了 27、39 和 20 倍。对于所分析的三种施用药物，骨型之间的相对分布具有相似的定性特征，所有三种药物的最大反应都来自肋骨。据作者所知，这是第一项在受控实验中使用与人类具有相似生理学的动物模型研究不同药物在各种分解骨骼组织中的分布的研究。这些数据对骨骼组织中法医药物测量的解释价值具有重要意义。
