Department of Chemistry, Tsinghua University, Beijing 100084, China.
Anal Chim Acta. 2011 Sep 19;702(1):81-6. doi: 10.1016/j.aca.2011.06.041. Epub 2011 Jun 28.
A highly selective and sensitive coumarin-based chemodosimeter 1 for Cu(2+) in water is reported in this work. 1 was designed and facilely synthesized by a one-step reaction with coumarin as a fluorophore and 2-picolinic acid as the binding moiety, which showed very week fluorescence in buffer solution, and its fluorescence was considerably enhanced by the addition of Cu(2+) at room temperature in 5 min. Mechanism study suggested that Cu(2+) promoted the hydrolysis of 1 via the catalytic sensing cycle, generating a highly fluorescent product 7-hydroxycoumarin with fluorescence signal greatly amplified. The probe exhibited remarkably selective fluorescence enhancement to Cu(2+) over other metal ions at 454 nm, with a detection limit of 35 nM Cu(2+). Under optimal condition, 1 was successfully used for the determination of Cu(2+) in fetal equine serum and two water samples.
本工作报道了一种在水中对 Cu(2+) 具有高选择性和高灵敏度的香豆素基比色传感器 1。1 通过以香豆素为荧光团和 2-吡啶甲酸为结合部分的一步反应设计并简便合成，在缓冲溶液中显示出非常弱的荧光，并且其荧光在室温下在 5 分钟内通过添加 Cu(2+)得到显著增强。机理研究表明，Cu(2+) 通过催化传感循环促进 1 的水解，生成具有荧光信号大大放大的高荧光产物 7-羟基香豆素。该探针在 454nm 处对 Cu(2+)具有显著的荧光增强选择性，检测限为 35nM Cu(2+)。在最佳条件下，1 成功用于测定胎马血清和两种水样中的 Cu(2+)。
