Suzuki N, Miyauchi T, Tomobe Y, Matsumoto H, Goto K, Masaki T, Fujino M
Tsukuba Research Laboratories, Takeda Chemical Industries, Ltd., Ibaraki, Japan.
Biochem Biophys Res Commun. 1990 Mar 30;167(3):941-7. doi: 10.1016/0006-291x(90)90614-s.
To investigate the possible involvement of endothelin-1 (ET-1), an endothelium-derived potent vasoconstrictor peptide, in the pathophysiology of hypertension, plasma ET-1 levels in 15-week-old spontaneously hypertensive rats (SHR) and DOCA-salt hypertensive rats were measured with a sandwich-type enzyme immunoassay. The vasocontractile effect of ET-1 in aortic helical preparations was significantly more sensitive in DOCA-salt hypertensive rats than in control sham-operated rats, but plasma levels of ET-1 did not differ between them. Plasma ET-1 levels in genetically hypertensive rats (SHR and stroke-prone SHR) were significantly lower than those in age-matched normotensive Wistar-Kyoto (WKY) rats. The plasma concentrations of big ET-1, a precursor of ET-1, in both SHR and SHR-SP were significantly lower than those of WKY, suggesting that the production of ET-1 is decreased in rats of genetic hypertension. Although the vascular reactivity to ET-1 increased in both DOCA-salt hypertensive and genetically hypertensive rats, present findings of the plasma ET-1 levels suggest that the role of ET-1 in the vascular control system may be different in DOCA-salt hypertensive rats and genetically hypertensive rats.
为研究内皮素 -1（ET -1）（一种内皮细胞源性强效血管收缩肽）在高血压病理生理学中的可能作用，采用夹心型酶免疫分析法测定了15周龄自发性高血压大鼠（SHR）和去氧皮质酮 -盐性高血压大鼠的血浆ET -1水平。在主动脉螺旋条制备物中，ET -1对去氧皮质酮 -盐性高血压大鼠的血管收缩作用比对假手术对照大鼠明显更敏感，但它们之间的血浆ET -1水平并无差异。遗传性高血压大鼠（SHR和易卒中SHR）的血浆ET -1水平显著低于年龄匹配的正常血压Wistar - Kyoto（WKY）大鼠。SHR和SHR - SP中ET -1的前体大ET -1的血浆浓度均显著低于WKY大鼠，提示遗传性高血压大鼠中ET -1的生成减少。尽管去氧皮质酮 -盐性高血压大鼠和遗传性高血压大鼠对ET -1的血管反应性均增加，但目前血浆ET -1水平的研究结果表明，ET -1在去氧皮质酮 -盐性高血压大鼠和遗传性高血压大鼠的血管控制系统中的作用可能不同。
