Irving R A, Toneguzzo F, Rhee S H, Hofmann T, Ghosh H P
Proc Natl Acad Sci U S A. 1979 Feb;76(2):570-4. doi: 10.1073/pnas.76.2.570.
Translation of mRNA encoding vesicular stomatitis virus envelope glycoprotein G by as membrane-free ribosomal extract obtained from HeLa cells yielded a nonglycosylated protein (G1 (Mr 63,000). In the presence of added microsomal membranes, G1 was converted to the glycosylated protein (G2 (Mr 67,000) which is inserted in the membrane vesicles as a transmembrane protein. Labeling with methionine donated by wheat germ initiator tRNA1Met showed that G1 but not G2 contains methionine in the NH2-terminal position. Determination of the NH2-terminal sequence of G1, G2, and G showed that a leader peptide of 16 amino acids is present in G1 but absent from the glycosylated proteins G2 and G. This leader peptide contains at least 62% hydrophobic amino acids and is removed presumably during insertion of G1 into the membrane.
从HeLa细胞获得的无膜核糖体提取物对编码水疱性口炎病毒包膜糖蛋白G的mRNA进行翻译，产生了一种非糖基化蛋白（G1，分子量63,000）。在添加微粒体膜的情况下，G1转化为糖基化蛋白（G2，分子量67,000），该蛋白作为跨膜蛋白插入膜泡中。用小麦胚芽起始tRNA1Met提供甲硫氨酸进行标记表明，G1在NH2末端位置含有甲硫氨酸，而G2不含。对G1、G2和G进行NH2末端序列测定表明，G1中存在一个16个氨基酸的前导肽，而糖基化蛋白G2和G中不存在。该前导肽至少含有62%的疏水氨基酸，可能在G1插入膜的过程中被去除。
