College of Chemistry and Environmental Science, Henan Normal University, Xinxiang, Henan 453007, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2011 Nov;82(1):247-52. doi: 10.1016/j.saa.2011.07.044. Epub 2011 Jul 23.
The binding properties of amino phosphate ester derivatives, compound 1 and 2 with calf thymus DNA (CT-DNA) were investigated by UV spectra, fluorescence spectra, molecular modeling and isothermal titration calorimetry (ITC). The intrinsic binding constants K(b) of compound 1 and 2 with CT-DNA were determined by fluorescence spectroscopy and ITC, respectively. The results indicated that the two compounds bind to CT-DNA with different binding affinity, which is in the order of compound 1>compound 2. At the same time, fluorescence spectra suggested that the mechanism of the binding of the two compounds to CT-DNA is a static enhancing type. According to the ethidium bromide displacement experiments, UV spectra, molecular modeling and ITC studies, it can be concluded that compound 1 and 2 are intercalators that can slide into the G-C rich region of CT-DNA. Furthermore, ITC data showed that compound/DNA binding is enthalpy controlled.
采用紫外可见吸收光谱、荧光光谱、分子模拟和等温热力学滴定（ITC）研究了氨基酸磷酸酯衍生物 1 和 2 与小牛胸腺 DNA（CT-DNA）的结合性质。通过荧光光谱法和 ITC 分别测定了化合物 1 和 2 与 CT-DNA 的固有结合常数 K(b)。结果表明，两种化合物与 CT-DNA 的结合亲和力不同，其顺序为化合物 1>化合物 2。同时，荧光光谱表明两种化合物与 CT-DNA 的结合机制为静态增强型。根据溴化乙锭置换实验、紫外可见吸收光谱、分子模拟和 ITC 研究，可以得出结论，化合物 1 和 2 是可以插入 CT-DNA 中富含 G-C 区域的嵌入剂。此外，ITC 数据表明化合物/DNA 结合是焓控制的。
