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为日本濒危水生植物品种芡(睡莲科)开发微卫星标记。

Development of microsatellite markers for Euryale ferox (Nymphaeaceae), an endangered aquatic plant species in Japan.

机构信息

Graduate School of Global Environmental Studies, Kyoto University, Yoshida-Honmachi, Sakyo-ku, Kyoto, 606-8501, Japan.

出版信息

Am J Bot. 2011 Aug;98(8):e233-5. doi: 10.3732/ajb.1100056.

Abstract

PREMISE OF THE STUDY

Microsatellite markers were developed to investigate genetic diversity and genetic structure of Euryale ferox, a vulnerable aquatic plant.

METHODS AND RESULTS

Ten microsatellite loci were isolated and characterized for this species. Eight loci showed polymorphisms with two or three alleles per locus. As for the polymorphic markers, the expected and observed heterozygosities ranged from 0.00 to 0.08 and 0.17 to 0.64, respectively.

CONCLUSIONS

The 10 microsatellite markers described here will be useful for investigating genetic diversity, genetic structure, and gene flow between populations of E. ferox.

摘要

研究前提

开发微卫星标记来研究芡实属濒危水生植物的遗传多样性和遗传结构。

方法和结果

为该物种分离和鉴定了 10 个微卫星基因座。8 个基因座显示每个基因座有两个或三个等位基因的多态性。对于多态性标记,期望和观察杂合度分别在 0.00 到 0.08 和 0.17 到 0.64 之间。

结论

本文描述的 10 个微卫星标记将有助于研究芡实属种群的遗传多样性、遗传结构和基因流。

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引用本文的文献

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