Kumar Nitish, Rani Shweta, Kuamr Gaurav, Kumari Swati, Singh Indu Shekhar, Gautam S, Choudhary Binod Kumar
Department of Biotechnology, School of Earth, Biological and Environmental Sciences, Central University of South Bihar, Gaya, Bihar, 824236, India.
Department of Life Science, School of Earth, Biological and Environmental Sciences, Central University of South Bihar, Gaya, Bihar, 824236, India.
J Biol Phys. 2019 Mar;45(1):1-12. doi: 10.1007/s10867-018-9511-x. Epub 2018 Oct 25.
The impact of gamma irradiation on growth and physiology of Euryale ferox was described in the present investigation. E. ferox is an underutilized aquatic food crop that grows in shallow-water bodies in lower Assam regions and north Bihar of India. The seeds of E. ferox were irradiated with different doses of gamma irradiation ranging from 0 to 500 Gy. It was observed that the germination and survival percentage was inhibited by increasing the irradiation dose. However, plants developed from seed exposed to an irradiation dose beyond 100 Gy did not survive more than 1 month. Further growth parameters (leaf size and number, number of thorns, root number and length, and number of flower and seeds) were also compared with respect to non-irradiated plants. Physiological parameters, viz. chlorophyll a, chlorophyll b, total chlorophyll, photosynthetic rate, transpiration rate, stomatal conductance, and intracellular CO content was higher in the irradiation population of E. ferox. Superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities were observed low in irradiated population of E. ferox. The proline and glycine betaine content was enhanced with increasing the irradiation dose. The present investigation explores the potential use of gamma rays in genetic improvement of E. ferox and improves understanding of the physiological responses inflicted by gamma irradiation.
本研究描述了γ射线辐照对芡实生长和生理的影响。芡实是一种未得到充分利用的水生粮食作物，生长在印度阿萨姆邦下游地区和比哈尔邦北部的浅水体中。用0至500 Gy的不同剂量γ射线辐照芡实种子。观察到发芽率和存活率随着辐照剂量的增加而受到抑制。然而，由暴露于超过100 Gy辐照剂量的种子发育而来的植株存活时间不超过1个月。还将进一步的生长参数（叶片大小和数量、刺的数量、根的数量和长度以及花和种子的数量）与未辐照植株进行了比较。芡实辐照群体的生理参数，即叶绿素a、叶绿素b、总叶绿素、光合速率、蒸腾速率、气孔导度和细胞内CO含量较高。在芡实辐照群体中观察到超氧化物歧化酶（SOD）和抗坏血酸过氧化物酶（APX）活性较低。脯氨酸和甘氨酸甜菜碱含量随着辐照剂量的增加而增加。本研究探索了γ射线在芡实遗传改良中的潜在用途，并增进了对γ射线辐照所引起的生理反应的理解。
