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以真养产碱杆菌PHB(-)4为宿主菌株，通过IV类聚羟基脂肪酸酯合成酶合成聚羟基脂肪酸酯（PHA）。

Polyhydroxyalkanoate (PHA) synthesis by class IV PHA synthases employing Ralstonia eutropha PHB(-)4 as host strain.

作者信息

Hyakutake Manami, Saito Yuta, Tomizawa Satoshi, Mizuno Kouhei, Tsuge Takeharu

机构信息

Department of Innovative and Engineered Materials, Tokyo Institute of Technology, Midori-ku, Yokohama, Japan.

出版信息

Biosci Biotechnol Biochem. 2011;75(8):1615-7. doi: 10.1271/bbb.110229. Epub 2011 Aug 7.

DOI:10.1271/bbb.110229
PMID:21821924
Abstract

Class IV polyhydroxyalkanoate (PHA) synthase from Bacillus cereus YB-4 (PhaRC(YB4)) or B. megaterium NBRC15308(T) (PhaRC(Bm)) was expressed in Ralstonia eutropha PHB(-)4 to compare the ability to produce PHA and the substrate specificity of PhaRCs. PhaRC(YB4) produced significant amounts of PHA and had broader substrate specificity than PhaRC(Bm).

摘要

将蜡样芽孢杆菌YB-4（PhaRC(YB4)）或巨大芽孢杆菌NBRC15308(T)（PhaRC(Bm)）的IV类聚羟基脂肪酸酯（PHA）合酶在真养产碱菌PHB(-)4中表达，以比较其产生PHA的能力和PHA合酶的底物特异性。PhaRC(YB4)产生了大量的PHA，并且比PhaRC(Bm)具有更广泛的底物特异性。

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