Nagai Hiroyuki, Goto Tsuyoshi, Takahashi Nobuyuki, Kusudoh Tatsuya, Deyashiki Yoshihiro, Esaka Yukihiro, Kawada Teruo
Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan.
Biosci Biotechnol Biochem. 2011;75(8):1485-9. doi: 10.1271/bbb.110192. Epub 2011 Aug 7.
A useful method employing liquid chromatography mass spectrometry (LC/MS) and a stable isotope was developed for simultaneous examination of major metabolism in adipocytes, de novo fatty acid synthesis, glycerol output, and glucose uptake with high sensitivity. The addition of thiazolidinediones, potent agonists of peroxisome proliferator-activated receptors-γ, for 10 d increased glucose uptake in a dose-dependent manner. Fatty acid (FA) synthesis increased at low concentrations of thiazolidinediones (TZDs) and decreased at high concentrations. It is important to assess adipocytes from various examples of metabolism, because each example of adipocyte metabolism is directly related to obesity or metabolic syndrome in various ways. The technique makes metabolic examination easier than conventional methods by means of radioisotopes and makes it possible to identify metabolites and to apply them in biomarker screening.
开发了一种采用液相色谱质谱联用（LC/MS）和稳定同位素的有用方法，用于以高灵敏度同时检测脂肪细胞中的主要代谢、脂肪酸从头合成、甘油输出和葡萄糖摄取。添加过氧化物酶体增殖物激活受体-γ的强效激动剂噻唑烷二酮10天，可使葡萄糖摄取呈剂量依赖性增加。在低浓度噻唑烷二酮（TZD）时脂肪酸（FA）合成增加，在高浓度时则减少。评估来自各种代谢实例的脂肪细胞很重要，因为每个脂肪细胞代谢实例都以各种方式与肥胖或代谢综合征直接相关。该技术比传统的放射性同位素方法更易于进行代谢检测，并且能够识别代谢物并将其应用于生物标志物筛选。
