通过光学显微镜和荧光显微镜分析拟南芥植株中质体的数量、大小和分布。
Analysis of plastid number, size, and distribution in Arabidopsis plants by light and fluorescence microscopy.
作者信息
Pyke Kevin
机构信息
Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Leicestershire, UK.
出版信息
Methods Mol Biol. 2011;774:19-32. doi: 10.1007/978-1-61779-234-2_2.
PMID:21822830
Abstract
Methods are described which allow one to observe chloroplasts in mesophyll cells from leaves of Arabidopsis, determine their number per cell, measure their area, and determine a value for chloroplast coverage inside mesophyll cells. Non-green plastids can also be imaged either by using staining, or by exploiting fluorescent proteins targeted to the plastid in non-green parts of the plant, such as the roots, in transgenic Arabidopsis.
摘要
本文描述了一些方法，这些方法可以让人观察拟南芥叶片叶肉细胞中的叶绿体，确定每个细胞中的叶绿体数量，测量其面积，并确定叶肉细胞内叶绿体覆盖度的值。对于非绿色质体，也可以通过染色，或者利用靶向植物非绿色部分（如转基因拟南芥的根）质体的荧光蛋白进行成像。
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