P1 溶菌酶 Lyz 无活性形式的¹H、¹³C 和¹⁵N 核磁共振归属
¹H, ¹³C and ¹⁵N NMR assignments of inactive form of P1 endolysin Lyz.
作者信息
Kashyap Maruthi, Jagga Zeenia, Das Bhaba Krishna, Arockiasamy Arulandu, Bhavesh Neel Sarovar
机构信息
Structural and Computational Biology group, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India.
出版信息
Biomol NMR Assign. 2012 Apr;6(1):87-9. doi: 10.1007/s12104-011-9331-4. Epub 2011 Aug 7.
Lysozyme (Lyz) encoded by phage P1 is required for host cell lysis upon infection. Lyz has a N-terminal Signal Anchor Release (SAR) domain, responsible for its secretion into the periplasm and for its accumulation in a membrane tethered inactive form. Here, we report sequence-specific (1)H, (13)C and (15)N resonance assignments for secreted inactive form of Lyz at pH 4.5.
噬菌体P1编码的溶菌酶（Lyz）在感染时是宿主细胞裂解所必需的。Lyz具有一个N端信号锚定释放（SAR）结构域，负责其分泌到周质中，并以膜结合的无活性形式积累。在这里，我们报告了pH 4.5时Lyz分泌无活性形式的序列特异性（1）H、（13）C和（15）N共振归属。
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