Liu Yang, Wu Yi-Ling, Cao Jia-Hui, Chen Dong-Feng, Zhou Jian-Hong, Deng Ru-Dong
Department of Anatomy, Preclinical Medical College, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.
Zhong Yao Cai. 2011 Mar;34(3):400-3.
To observe the inhibitive effects of Plastrum testudinis Extracts (PTE) on 6-Hydroxydopamine (6-OHDA) induced PC12 cells apoptosis and explore its mechanism.
PC12 apoptosis model was established by serum starvation and damaged for 24 hours. The cells were randomly divided into four groups:control group, 6-OHDA group, PTE 3, 30 microg/mL group. Cell optical density was determined by MTT; Ratio of cell apoptosis was examined by Annexin V/PI double stain flow cytometry (FCM), and Western blot was applied to detect the BCL-X/L expression.
MTT and FCM analysis demonstrated that PTE can elevate PC12 cells viability and reduce their apoptotic ratio in a dose dependent manner. Western blot showed that PTE promoted the expression of BCL-X/L.
PTE can inhibit the apoptosis of PC12 induced by 6-OHDA in a dose dependent manner, and its mechanism maybe associated partially with up-regulating BCL-X/L signaling pathway.
观察龟板提取物（PTE）对6-羟基多巴胺（6-OHDA）诱导的PC12细胞凋亡的抑制作用，并探讨其机制。
通过血清饥饿建立PC12细胞凋亡模型并损伤24小时。将细胞随机分为四组：对照组、6-OHDA组、PTE 3、30μg/mL组。采用MTT法测定细胞光密度；采用Annexin V/PI双染流式细胞术（FCM）检测细胞凋亡率，并用Western blot检测BCL-X/L表达。
MTT和FCM分析表明，PTE能以剂量依赖性方式提高PC12细胞活力，降低其凋亡率。Western blot显示PTE促进了BCL-X/L的表达。
PTE能以剂量依赖性方式抑制6-OHDA诱导的PC12细胞凋亡，其机制可能部分与上调BCL-X/L信号通路有关。
