黄连素对高脂高盐高果糖饮食诱导的高血压肾损伤大鼠的作用及机制

[Effects and mechanism of berberine on the hypertensive renal injury rats induced by enriched high fat-salt-fructose diet].

作者信息

Li Hong-Bin, Cai Ying, Qi Chun-Li, Lv Jun-Hua

机构信息

The First Affiliated Hospital, Guangzhou 510632, China.

出版信息

Zhong Yao Cai. 2011 Mar;34(3):412-5.

PMID:21823461

Abstract

OBJECTIVE

To study the effects of berberine on hypertensive renal injury model in rats fed by enriched high fat-salt-fructose diet .

METHODS

hypertensive renal injury model was esteblished by feeding enriched high fat-salt-fructose diet for 8 weeks. On the basis of animal blood pressure, hypertensive rats were randomly divided into model group (10 rats, distilled water), captopril group (10 rats,25 mg/kg), berberine high dose group (10 rats, 300 mg/kg) and low dose group (10 rats, 100 mg/kg). These rats were fed by enriched high fat-salt-fructose diet and treated by intragastric administration with drugs for 4 weeks. And normal control group (10 rats) was set Blood pressure was determined at 0, 4, 8, 10, 12 weekend,and after 4 weeks of drugs treatment, getting urine to determine urine protein, taking blood serum to determine blood urea nitrogen, serum creatinine,GHb,MDA and activity of SOD. The content of H2O2 and GSH-Px and activity of CAT in kidney tissues were determined also.

RESULTS

Compared with normal control group, blood pressure, urine protein, blood urea nitrogen, serum creatinine and MDA and GHb in serum of model rats obviously increased (P < 0.01), the activity of SOD decreased (P < 0.01), higher content of H2O2 and lower content of GSH-Px and activity of CAT (P < 0.01) in the kidney tissues. Treated with berberine for 4 weeks, elevated blood pressure and heightened levels of urine protein, blood urea nitrogen and serum creatinine in model rats were depressed significantly (P < 0.01), and elevated the activity of SOD, lowed the levels of MDA and GHb in blood serum (P < 0.01). At the same time, berberine increased the activities of GSH-Px and CAT (P < 0.01) and slightly lowed the content of H2O2 in the kidney tissues.

CONCLUSION

Berberine has protecting effects on the hypertensive renal impairment model rats fed by enriched high fat-salt-fructose diet, which are concerned with elevated antioxidant capability in body and kidney tissues.

摘要

目的

研究黄连素对高脂高盐高果糖饮食喂养的大鼠高血压肾损伤模型的影响。

方法

通过高脂高盐高果糖饮食喂养8周建立高血压肾损伤模型。根据动物血压情况，将高血压大鼠随机分为模型组（10只，蒸馏水）、卡托普利组（10只，25mg/kg）、黄连素高剂量组（10只，300mg/kg）和低剂量组（10只，100mg/kg）。这些大鼠继续高脂高盐高果糖饮食喂养，并通过灌胃给药治疗4周。同时设立正常对照组（10只）。在0、4、8、10、12周末测定血压，药物治疗4周后，留取尿液测定尿蛋白，采集血清测定血尿素氮、血清肌酐、糖化血红蛋白、丙二醛和超氧化物歧化酶活性。同时测定肾组织中过氧化氢含量、谷胱甘肽过氧化物酶含量和过氧化氢酶活性。

结果

与正常对照组相比，模型大鼠血压、尿蛋白、血尿素氮、血清肌酐、血清丙二醛和糖化血红蛋白明显升高（P<0.01），超氧化物歧化酶活性降低（P<0.01），肾组织中过氧化氢含量升高、谷胱甘肽过氧化物酶含量和过氧化氢酶活性降低（P<0.01）。黄连素治疗4周后，模型大鼠升高的血压及升高的尿蛋白、血尿素氮和血清肌酐水平均明显降低（P<0.01），超氧化物歧化酶活性升高，血清丙二醛和糖化血红蛋白水平降低（P<0.01）。同时，黄连素提高了肾组织中谷胱甘肽过氧化物酶和过氧化氢酶活性（P<0.01），并使肾组织中过氧化氢含量略有降低。