Tian Jun-Long, Cai Pei-Ling, Xia Xiao-Qiang, Li Fang-Hua, Wang De-Hua, Chen Mi-Na
State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2011 May;42(3):422-6.
To generate the cancer stem cells (CSCs) specific protein CD133 polyclonal antibody for the study of the biological characteristics of CSCs in tumor tissues and CSCs screening for the mouse model.
The extracellular peptide of the human CD133 was injected into rabbits to generate polyclonal antibody which was used for glioblastoma(GBM) Western blot and immunohistochemistry.
The CD133 antiserum we made could detect both overexpressed myc-CD133 and endogenous CD133 efficiently by Western blot. Immunohistochemistry indicated that the CD133 polyclonal antibody can label CSCs in GBM sections.
High efficient and specific CD133 antibody was generated successfully and could be used to label CSCs in tumor sections and screen CSCs for the mouse model.
制备癌症干细胞（CSCs）特异性蛋白CD133多克隆抗体，用于研究肿瘤组织中CSCs的生物学特性以及在小鼠模型中筛选CSCs。
将人CD133的细胞外肽段注射到兔子体内以产生多克隆抗体，该抗体用于胶质母细胞瘤（GBM）的蛋白质免疫印迹和免疫组织化学分析。
我们制备的CD133抗血清通过蛋白质免疫印迹能够有效检测过表达的myc-CD133和内源性CD133。免疫组织化学表明，CD133多克隆抗体可标记GBM切片中的CSCs。
成功制备了高效且特异性的CD133抗体，可用于标记肿瘤切片中的CSCs以及在小鼠模型中筛选CSCs。
