巴西人类感染伤寒血清型鼠伤寒沙门氏菌 DT193 菌株中 R 型 ACSSuT 的流行情况。

Prevalence of R-type ACSSuT in strains of Salmonella serovar Typhimurium DT193 isolated from human infections in Brazil.

机构信息

Laboratório de Enterobactérias, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.

出版信息

Rev Panam Salud Publica. 2011 Jun;29(6):387-92.

Abstract

OBJECTIVE

To determine the prevalence of resistance to ampicillin, chloramphenicol, streptomycin, sulphonamides, and tetracyclines (ACSSuT) in Salmonella serovar Typhimurium definitive [phage] type (DT) 193 strains isolated from human sources over the last four decades.

METHODS

From 2008 to 2010, 553 DT193 isolates out of 810 human-origin Salmonella ser. Typhimurium phage-typed strains isolated from the 1970s through 2008 were selected and tested for ACSSuT resistance: 91 strains isolated during the 1970s, 65 from the 1980s, 70 from the 1990s, and 327 from 2000-2008. Resistance profiles were determined using the disk diffusion method.

RESULTS

An antimicrobial susceptibility assay indicated 20.9%, or 116, of all isolates tested were ACSSuT-resistant, 52.0% (287) were resistant to one or more drugs in the ACSSuT profile, and 27.1% (150) were nonresistant (susceptible to antimicrobials). Based on the assay, overall antimicrobial resistance was extremely high in the 1970s (affecting 99.0% of isolates from that period) and remained high during the 1980s, when 95.4% of isolates had some type of antimicrobial resistance and incidence of Salmonella ser. Typhimurium DT193 R-type ACSSuT increased to 73.8%. R-type ACSSuT dropped to 27.1% (19 isolates) during the 1990s, and to 5.2% (17) during 2000-2008, despite a substantial increase in the number of isolates tested (397 versus 204, 111, and 98, respectively, for the previous three decades).

CONCLUSIONS

Although prevalence of Salmonella ser. Typhimurium DT193 R-type ACSSuT in Brazil has decreased since the 1970s, ACSSuT resistance markers continue to circulate. Therefore, continuous surveillance should be conducted to evaluate the occurrence of Salmonella ser. Typhimurium DT193 and its antimicrobial resistance.

摘要

目的

确定过去四十年间从人类源分离的肠炎沙门氏菌 Typhimurium 定型[噬菌体]型（DT）193 菌株对氨苄青霉素、氯霉素、链霉素、磺胺类药物和四环素（ACSSuT）的耐药率。

方法

2008 年至 2010 年，从 1970 年代至 2008 年分离的 810 株肠炎沙门氏菌 Typhimurium 噬菌体型菌株中选择 553 株 DT193 分离株进行 ACSSuT 耐药性检测：70 年代分离的菌株 91 株，80 年代分离的菌株 65 株，90 年代分离的菌株 70 株，2000-2008 年分离的菌株 327 株。采用纸片扩散法测定耐药谱。

结果

药敏试验表明，20.9%（116 株）的所有受试分离株均对 ACSSuT 耐药，52.0%（287 株）对 ACSSuT 耐药谱中的一种或多种药物耐药，27.1%（150 株）非耐药（对所有抗菌药物敏感）。根据该试验，70 年代的整体抗菌药物耐药性极高（影响该时期 99.0%的分离株），80 年代的耐药性仍然很高，当时 95.4%的分离株具有某种类型的抗菌药物耐药性，肠炎沙门氏菌 Typhimurium DT193 R 型 ACSSuT 的发生率增加到 73.8%。90 年代 R 型 ACSSuT 下降至 27.1%（19 株），2000-2008 年下降至 5.2%（17 株），尽管测试的分离株数量大幅增加（分别为 397 株、204 株、111 株和 98 株）。