Department of Nuclear Medicine, Faculty of Medicine, Ataturk University, 25240 Erzurum, Turkey.
Nucl Med Biol. 2012 Jan;39(1):161-5. doi: 10.1016/j.nucmedbio.2011.06.002. Epub 2011 Aug 9.
The aim of the study was to evaluate the inhibitory effects of thallium-201 ((201)Tl) solution on human erythrocyte glutathione reductase (GR) activity.
Erythrocyte GR was initially purified by 2',5'-adenosine diphosphate Sepharose-4B affinity and Sephadex G-200 gel filtration chromatography. The purification was monitored by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, which showed a single band for the final enzyme preparation. The in vitro and in vivo effects of the (201)Tl solution including Tl(+), Fe(+3) and Cu(+2) metals and the in vitro effects of the radiation effect of the (201)Tl solution and nonradioactive Tl(+), Fe(+3) and Cu(+2) metals on human erythrocyte GR enzyme were studied. Enzyme activity was determined with the Beutler method at 340 nm using a spectrophotometer. All purification procedures were carried out at (+)4 °C.
Glutathione reductase was purified 2033-fold at a yield of 28.17%. (201)Tl solution and radiation exposure had inhibitory effects on the enzyme activity. Besides, effects of nonradioactive Tl(+), Fe(+3) and Cu(+2) were studied on enzyme activity in vitro. Furthermore, seven human patients were also used for in vivo studies of (201)Tl solution.
It was detected in in vitro and in vivo studies that the human erythrocyte GR enzyme is inhibited due to the radiation effect of (201)Tl solution.
本研究旨在评估铊-201((201)Tl)溶液对人红细胞谷胱甘肽还原酶(GR)活性的抑制作用。
首先通过 2',5'-二磷酸腺苷琼脂糖-4B 亲和和葡聚糖凝胶 G-200 凝胶过滤层析法对红细胞 GR 进行初步纯化。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行监测，最终酶制剂显示出单一的带。研究了(201)Tl 溶液中的 Tl(+)、Fe(+3)和 Cu(+2)金属的体外和体内效应，以及(201)Tl 溶液的辐射效应和非放射性 Tl(+)、Fe(+3)和 Cu(+2)金属的体外对人红细胞 GR 酶的影响。使用分光光度计在 340nm 处使用 Beutler 法测定酶活性。所有纯化程序均在(+)4°C 下进行。
谷胱甘肽还原酶的纯化倍数为 2033 倍，收率为 28.17%。(201)Tl 溶液和辐射暴露对酶活性具有抑制作用。此外，还研究了非放射性 Tl(+)、Fe(+3)和 Cu(+2)对体外酶活性的影响。此外，还对 7 名人类患者进行了(201)Tl 溶液的体内研究。
在体外和体内研究中都检测到，由于(201)Tl 溶液的辐射效应，人红细胞 GR 酶受到抑制。
