Swire Institute of Marine Science and School of Biological Sciences, The University of Hong Kong.
Comp Biochem Physiol Part D Genomics Proteomics. 2011 Sep;6(3):310-21. doi: 10.1016/j.cbd.2011.07.001. Epub 2011 Jul 21.
The majority of benthic marine invertebrates have a complex life cycle, during which the pelagic larvae select a suitable substrate, attach to it, and then metamorphose into benthic adults. Anthropogenic ocean acidification (OA) is postulated to affect larval metamorphic success through an altered protein expression pattern (proteome structure) and post-translational modifications. To test this hypothesis, larvae of an economically and ecologically important barnacle species Balanus amphitrite, were cultured from nauplius to the cyprid stage in the present (control) and in the projected elevated concentrations of CO(2) for the year 2100 (the OA treatment). Cyprid response to OA was analyzed at the total proteome level as well as two protein post-translational modification (phosphorylation and glycosylation) levels using a 2-DE based proteomic approach. The cyprid proteome showed OA-driven changes. Proteins that were differentially up or down regulated by OA come from three major groups, namely those related to energy-metabolism, respiration, and molecular chaperones, illustrating a potential strategy that the barnacle larvae may employ to tolerate OA stress. The differentially expressed proteins were tentatively identified as OA-responsive, effectively creating unique protein expression signatures for OA scenario of 2100. This study showed the promise of using a sentinel and non-model species to examine the impact of OA at the proteome level.
大多数底栖海洋无脊椎动物具有复杂的生命周期，在此期间，浮游幼虫选择合适的基质，附着在基质上，然后变态为底栖成虫。有人假设人为海洋酸化 (OA) 通过改变蛋白质表达模式（蛋白质组结构）和翻译后修饰来影响幼虫变态成功。为了验证这一假设，本文以经济和生态上重要的藤壶物种藤壶（Balanus amphitrite）的幼虫为研究对象，在当前（对照）和预计的 2100 年 CO2 升高浓度下（OA 处理），从无节幼体培养到幼体阶段。使用基于 2-DE 的蛋白质组学方法，在总蛋白质组水平以及两种蛋白质翻译后修饰（磷酸化和糖基化）水平上分析了幼体对 OA 的反应。幼体蛋白质组显示出 OA 驱动的变化。OA 上调或下调的蛋白质来自三个主要组，即与能量代谢、呼吸和分子伴侣相关的蛋白质，这表明藤壶幼虫可能采用的一种潜在策略来耐受 OA 应激。差异表达的蛋白质被暂时鉴定为 OA 反应蛋白，有效地为 2100 年的 OA 情景创造了独特的蛋白质表达特征。本研究表明，使用哨兵和非模式物种在蛋白质组水平上研究 OA 影响具有潜力。
