The Swire Institute of Marine Science and School of Biological Sciences, The University of Hong Kong, Hong Kong.
Mar Pollut Bull. 2012 Oct;64(10):2160-7. doi: 10.1016/j.marpolbul.2012.07.043. Epub 2012 Aug 24.
Most calcifying organisms show depressed metabolic, growth and calcification rates as symptoms to high-CO(2) due to ocean acidification (OA) process. Analysis of the global expression pattern of proteins (proteome analysis) represents a powerful tool to examine these physiological symptoms at molecular level, but its applications are inadequate. To address this knowledge gap, 2-DE coupled with mass spectrophotometer was used to compare the global protein expression pattern of oyster larvae exposed to ambient and to high-CO(2). Exposure to OA resulted in marked reduction of global protein expression with a decrease or loss of 71 proteins (18% of the expressed proteins in control), indicating a wide-spread depression of metabolic genes expression in larvae reared under OA. This is, to our knowledge, the first proteome analysis that provides insights into the link between physiological suppression and protein down-regulation under OA in oyster larvae.
大多数钙化生物由于海洋酸化（OA）过程而表现出代谢、生长和钙化率降低的症状。对蛋白质的全球表达模式（蛋白质组分析）的分析代表了在分子水平上检查这些生理症状的有力工具，但它的应用还不够充分。为了解决这一知识差距，我们使用 2-DE 与质谱仪相结合的方法比较了暴露于环境和高 CO2 条件下的牡蛎幼虫的全球蛋白质表达模式。暴露于 OA 导致全球蛋白质表达明显减少，71 种蛋白质减少或丢失（对照中表达蛋白质的 18%），表明在 OA 下培养的幼虫中代谢基因表达广泛受到抑制。据我们所知，这是首次在牡蛎幼虫中进行蛋白质组分析，深入了解 OA 下生理抑制与蛋白质下调之间的联系。
