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内吞作用对于有丝分裂酵母减数分裂中突触蛋白 1 同源物的动态易位是必不可少的。

Endocytosis is essential for dynamic translocation of a syntaxin 1 orthologue during fission yeast meiosis.

机构信息

Department of Biology, Graduate School of Science, Osaka City University, Osaka 558-8585, Japan.

出版信息

Mol Biol Cell. 2011 Oct;22(19):3658-70. doi: 10.1091/mbc.E11-03-0255. Epub 2011 Aug 10.

DOI:10.1091/mbc.E11-03-0255
PMID:21832151
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3183020/
Abstract

Syntaxin is a component of the target soluble N-ethylmaleimide-sensitive factor attachment protein receptor complex, which is responsible for fusion of membrane vesicles at the target membrane. The fission yeast syntaxin 1 orthologue Psy1 is essential for both vegetative growth and spore formation. During meiosis, Psy1 disappears from the plasma membrane (PM) and dramatically relocalizes on the nascent forespore membrane, which becomes the PM of the spore. Here we report the molecular details and biological significance of Psy1 relocalization. We find that, immediately after meiosis I, Psy1 is selectively internalized by endocytosis. In addition, a meiosis-specific signal induced by the transcription factor Mei4 seems to trigger this internalization. The internalization of many PM proteins is facilitated coincident with the initiation of meiosis, whereas Pma1, a P-type ATPase, persists on the PM even during the progression of meiosis II. Ergosterol on the PM is also important for the internalization of PM proteins in general during meiosis. We consider that during meiosis in Schizosaccharomyces pombe cells, the characteristics of endocytosis change, thereby facilitating internalization of Psy1 and accomplishing sporulation.

摘要

yntaxin 是靶可溶性 N-乙基马来酰亚胺敏感因子附着蛋白受体复合物的一个组成部分，负责靶膜上囊泡的融合。裂殖酵母 syntaxin 1 同源物 Psy1 对于营养生长和孢子形成都是必需的。在减数分裂过程中， Psy1 从质膜（PM）消失，并在新形成的前孢子膜上显著重新定位，该膜成为孢子的 PM。在这里，我们报告 Psy1 重定位的分子细节和生物学意义。我们发现，在减数分裂 I 之后， Psy1 被内吞作用选择性地内化。此外，由转录因子 Mei4 诱导的减数分裂特异性信号似乎触发了这种内化。许多 PM 蛋白的内化伴随着减数分裂的开始而发生，而 Pma1，一种 P 型 ATP 酶，即使在减数分裂 II 的进行过程中，也仍然存在于 PM 上。PM 上的麦角固醇对于减数分裂过程中 PM 蛋白的内化通常也是重要的。我们认为，在裂殖酵母细胞的减数分裂过程中，内吞作用的特性发生变化，从而促进 Psy1 的内化并完成孢子形成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/2d55a00ccc5c/3658fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/69dd3216c346/3658fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/2cee6167ad11/3658fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/1e82daf1cbeb/3658fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/dbab6bc3d6ab/3658fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/c6a35d645ff5/3658fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/ef7c2a0dc355/3658fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/f392eff93a27/3658fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/1259f433730d/3658fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/b7f175bf613c/3658fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/2d55a00ccc5c/3658fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/69dd3216c346/3658fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/2cee6167ad11/3658fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/1e82daf1cbeb/3658fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/dbab6bc3d6ab/3658fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/c6a35d645ff5/3658fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/ef7c2a0dc355/3658fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/f392eff93a27/3658fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/1259f433730d/3658fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/b7f175bf613c/3658fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b816/3183020/2d55a00ccc5c/3658fig10.jpg

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