Department of Molecular and Experimental Medicine, The Scripps Research Institute, LA Jolla, CA, USA.
Blood. 2011 Sep 29;118(13):3694-7. doi: 10.1182/blood-2011-03-342113. Epub 2011 Aug 10.
Maintenance of a reducing redox balance is a critical physiologic function of red cells (RBC) that can be perturbed in variety of RBC pathologies. Here we describe a new approach to evaluate in vivo RBC redox status using a redox sensitive GFP (roGFP2) sensor under control of a β-globin mini-promoter, directing expression specifically to erythroid cells. RoGFP2 expressing RBCs demonstrate ratiometric and reversible shifts in fluorescence on exposure to oxidants and reductants. We demonstrate that roGFP2 expressing RBC can be used to monitor thiol redox status during in vitro phenylhydrazine treatment and over the course of in vivo RBC aging, where a shift to a more oxidized state is observed in older cells. Thus, roGFP2 transgenic mice are a new and versatile tool that can be used to probe how RBC redox status responds in the context of drug therapy, physiologic stressors and pathologic states.
维持还原氧化平衡是红细胞（RBC）的一项关键生理功能，在各种 RBC 病理情况下都会受到干扰。在这里，我们描述了一种使用氧化还原敏感 GFP（roGFP2）传感器评估体内 RBC 氧化还原状态的新方法，该传感器受β-球蛋白微启动子控制，专门指导红细胞表达。表达 roGFP2 的 RBC 在暴露于氧化剂和还原剂时表现出比率和可逆的荧光偏移。我们证明，表达 roGFP2 的 RBC 可用于监测体外苯肼处理过程中的巯基氧化还原状态，并在体内 RBC 衰老过程中进行监测，在衰老细胞中观察到向更氧化状态的转变。因此，roGFP2 转基因小鼠是一种新的多功能工具，可用于研究 RBC 氧化还原状态在药物治疗、生理应激和病理状态下的反应方式。
