Pei Zhiguo, Qin Lingsong, Zhang Zhihong, Zeng Shaoqun, Huang Zhen-Li
Biomed Opt Express. 2011 Aug 1;2(8):2117-25. doi: 10.1364/BOE.2.002117. Epub 2011 Jul 1.
Characterizing the photoactivation performance of optical highlighter fluorescent proteins is crucial to the realization of photoactivation localization microscopy. In contrast to those fluorescence-based approaches that require complex data processing and calibration procedures, here we report a simple and quantitative alternative, which relies on the measurement of small absorption spectra changes over time with a fiber-optic system. Using Dronpa as a representative highlighter protein, we have investigated the capacity of this system in monitoring the fast photoactivation process.
表征光学标记荧光蛋白的光激活性能对于实现光激活定位显微镜至关重要。与那些需要复杂数据处理和校准程序的基于荧光的方法不同，在这里我们报告了一种简单且定量的替代方法，该方法依赖于使用光纤系统测量随时间变化的小吸收光谱变化。以Dronpa作为代表性的标记蛋白，我们研究了该系统监测快速光激活过程的能力。
