School of Basic Medicine, Henan College of Traditional Chinese Medicine, Zhengzhou, China.
Curr Microbiol. 2011 Oct;63(4):387-91. doi: 10.1007/s00284-011-9991-4. Epub 2011 Aug 11.
Helicobacter pylori (H. pylori) has been identified as the main pathogenic factors of chronic gastritis and peptic ulcer, and the Class I carcinogen of gastric cancer by WHO. Vaccine has become the most effective measure to prevent and cure H. pylori infection. The UreB is the most effective and common immunogen of all strains of H. pylori and may stimulate the immunoresponse protecting the human body against the challenge of H. pylori. UreB antigen gene was cloned into the binary vector pBI121 which contains a seed-specific promoter Oleosin of peanut and a kanamycin resistance gene, and then UreB gene was transformed into peanut embryo leaflets by Agrobacter-mediated method. The putative transgenic plants were examined for the presence of UreB in the nuclear genome of peanut plants by PCR analysis. Expression of UreB gene in plants was identified by RT-PCR and Western blot analysis. These results suggest that the UreB transgenic peanut can be potentially used as an edible vaccine for controlling H. pylori.
幽门螺杆菌（H. pylori）已被世界卫生组织（WHO）确定为慢性胃炎和消化性溃疡的主要致病因素，以及胃癌的 I 类致癌物。疫苗已成为预防和治疗 H. pylori 感染的最有效措施。UreB 是 H. pylori 所有菌株中最有效和最常见的免疫原，可能会刺激免疫反应，保护人体免受 H. pylori 的侵袭。UreB 抗原基因被克隆到含有花生种子特异性启动子 Oleosin 和卡那霉素抗性基因的二元载体 pBI121 中，然后通过农杆菌介导的方法将 UreB 基因转化到花生胚叶片中。通过 PCR 分析检测花生植株核基因组中 UreB 的存在，来鉴定拟转基因植物。通过 RT-PCR 和 Western blot 分析鉴定 UreB 基因在植物中的表达。这些结果表明，UreB 转基因花生可作为控制 H. pylori 的口服疫苗。
