Perry D Alexander, Morrison Hilary G, Adam Rodney D
Infectious Disease Section, College of Medicine, University of Arizona, 1501 N. Campbell, Tucson, AZ 85724-5039, USA.
Mol Biochem Parasitol. 2011 Dec;180(2):112-4. doi: 10.1016/j.molbiopara.2011.07.008. Epub 2011 Jul 30.
The Giardia lamblia genome consists of 12 Mb divided among 5 chromosomes ranging in size from approximately 1 to 4 Mb. The assembled contigs of the genotype A1 isolate, WB, were previously mapped along the 5 chromosomes on the basis of hybridization of plasmid clones representing the contigs to chromosomes separated by PFGE. In the current report, we have generated an MluI optical map of the WB genome to improve the accuracy of the physical map. This has allowed us to correct several assembly errors and to better define the extent of the subtelomeric regions that are not included in the genome assembly.
蓝氏贾第鞭毛虫基因组由12兆碱基组成,分布在5条染色体上,大小约为1至4兆碱基。基因型A1分离株WB的组装重叠群先前已根据代表重叠群的质粒克隆与通过脉冲场凝胶电泳分离的染色体的杂交结果,定位到这5条染色体上。在本报告中,我们生成了WB基因组的MluI光学图谱,以提高物理图谱的准确性。这使我们能够纠正几个组装错误,并更好地确定基因组组装中未包含的亚端粒区域的范围。