一株K2中性酿酒酵母菌株含有一个变异的K2 M基因组。

A K2 neutral Saccharomyces cerevisiae strain contains a variant K2 M genome.

作者信息

Wingfield B D, Southgate V J, Pretorius I S, van Vuuren H J

机构信息

Department of Microbiology, University of Stellenbosch, South Africa.

出版信息

Yeast. 1990 Mar-Apr;6(2):159-69. doi: 10.1002/yea.320060210.

DOI:10.1002/yea.320060210

PMID:2183523

Abstract

K2 neutral strain Saccharomyces cerevisiae USM12 was identified and characterized. This strain carried an M double-stranded RNA (dsRNA) genome encoding for resistance to K2 toxin. The M dsRNA was larger than the K2 killer yeast M dsRNA and homoduplex analysis of denatured and reannealed K2 neurtal M dsRNA revealed an inverted duplication. Heteroduplex analysis showed that two thirds of the K2 M genome had homology with the K2 neutral M genome. Hybridization showed that the USM12 M dsRNA had significant homology with the K2 M dsRNA. Protein profiles of extracellular proteins from USM12 and a cured strain indicated that USM12 did not secrete any toxin. This is the first time that a K2 neutral yeast strain has been characterized.

摘要

鉴定并表征了K2中性菌株酿酒酵母USM12。该菌株携带一个M双链RNA（dsRNA）基因组，编码对K2毒素的抗性。M dsRNA比K2杀伤酵母M dsRNA大，对变性和复性后的K2中性M dsRNA进行同源双链分析，发现存在反向重复。异源双链分析表明，K2 M基因组的三分之二与K2中性M基因组具有同源性。杂交显示，USM12 M dsRNA与K2 M dsRNA具有显著同源性。来自USM12和一个治愈菌株的细胞外蛋白质的蛋白质谱表明，USM12不分泌任何毒素。这是首次对K2中性酵母菌株进行表征。