Department of Oral Maxillofacial Surgery, National Taiwan University Hospital, Taipei, Taiwan, Republic of China.
Head Neck. 2011 Sep;33(9):1301-8. doi: 10.1002/hed.21607. Epub 2010 Nov 10.
T helper 17 (Th17) and regulatory T cells share plasticity in the expression of interleukin (IL)-17 and forkhead box P3 (FOXP3), but their mutual presence in human diseases is unclear.
IL-17 and FOXP3 were analyzed by immunohistostaining and flow cytometry. The cytokine milieu was analyzed by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR).
Oral squamous cell carcinoma expresses high levels of IL-1β, IL-6, and transforming growth factor (TGF)-β. A unique subset of FOXP3(+) IL-17-producing CD4(+) T cells was consistently identified in tumor-infiltrating lymphocytes from advanced stages of cancer, but not in the circulation, at a frequency of 0.5% to 5.5 % of total CD4(+) T and positively correlated with the frequency of IL-17(+)FOXP3(-) T cells. The IL-17(+)FOXP3(+) T cells express CCR6 and suppress the proliferation of autologous CD4(+) CD25(-) responder T-cells in vitro.
The prevalence of IL-17-producing FOXP3(+) CD4(+) tumor infiltrating lymphocytes is increased in oral squamous cell carcinoma.
辅助性 T 细胞 17（Th17）和调节性 T 细胞在白细胞介素（IL）-17 和叉头框 P3（FOXP3）的表达上具有可塑性，但它们在人类疾病中的共同存在尚不清楚。
通过免疫组织化学染色和流式细胞术分析 IL-17 和 FOXP3。通过定量逆转录-聚合酶链反应（RT-PCR）分析细胞因子微环境。
口腔鳞状细胞癌表达高水平的 IL-1β、IL-6 和转化生长因子（TGF）-β。在癌症晚期肿瘤浸润淋巴细胞中始终鉴定出一种独特的 FOXP3(+)IL-17 产生 CD4(+)T 细胞亚群，但在循环中不存在，频率为总 CD4(+)T 的 0.5%至 5.5%，与 IL-17(+)FOXP3(-)T 细胞的频率呈正相关。IL-17(+)FOXP3(+)T 细胞表达 CCR6，并在体外抑制自身 CD4(+)CD25(-)应答 T 细胞的增殖。
在口腔鳞状细胞癌中，产生 IL-17 的 FOXP3(+)CD4(+)肿瘤浸润淋巴细胞的发生率增加。
