Krsková Zuzana, Martin Jan, Dusek Jaroslav
Department of Pharmacognosy, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Czech Republic.
Ceska Slov Farm. 2011 Jun;60(3):125-31.
This thesis deals with testing of inhibition activity beta-carboline alkaloids on activity of enzymes acetylcholinesterase (ACHE) and butyrylcholinesterase (BUCHE) using test "Fast Blue B salt" at TLC desk and Ellman's test using spectrophotometer. It was also investigated how dimethylsulfoxide used as a solvent in combination with water affects activity of enzymes and alkaloids. Results show harmine in form of base and salt in water and in mixture of DMSO and water has the hightest inhibition activity on ACHE using eserine as reference substance. Harmalol in form of salt in water and harmine in form of base and salt in mixture of DMSO and water has the hightest activity on BUCHE. It was find out that DMSO considerably affects activity of enzymes and alkaloids.
本论文采用薄层色谱台上的“固蓝B盐”试验以及使用分光光度计的埃尔曼试验，研究了β-咔啉生物碱对乙酰胆碱酯酶（ACHE）和丁酰胆碱酯酶（BUCHE）活性的抑制作用。还研究了用作溶剂的二甲基亚砜与水混合后对酶和生物碱活性的影响。结果表明，以水以及二甲基亚砜与水的混合物中的碱和盐形式存在的哈尔明，以毒扁豆碱作为参比物质时，对ACHE具有最高的抑制活性。以水合盐形式存在的哈尔醇以及以二甲基亚砜与水的混合物中的碱和盐形式存在的哈尔明，对BUCHE具有最高活性。研究发现，二甲基亚砜对酶和生物碱的活性有显著影响。
