An investigation on the fluorescence quenching of 9-aminoacridine by certain pyrimidines.

The fluorescence quenching of 9-aminoacridine by certain substituted uracils in water was studied using absorption, steady state and time resolved measurements. The bimolecular quenching rate constants (k(q)), binding constant K and number of binding sites (n) were calculated based on the fluorescence quenching data. The free energy change (ΔG(et)) for electron transfer process was calculated by Rehm-Weller equation. From lifetime measurement we observed that the quenching was mainly due to static mechanism involving ground state complex formation.