Department of Metallurgical and Materials Engineering, Atilim University, Incek, Golbasi, Ankara, Turkey.
Int J Biol Macromol. 2011 Nov 1;49(4):838-46. doi: 10.1016/j.ijbiomac.2011.07.022. Epub 2011 Aug 4.
In this study, natural biodegradable polysaccharide, chitosan, and synthetic biodegradable polymer, poly(ɛ-caprolactone) (PCL) were used to prepare 3D, hybrid polymeric tissue scaffolds (PCL/chitosan blend and PCL/chitosan/PCL layer by layer scaffolds) by using the electrospinning technique. The hybrid scaffolds were developed through HA addition to accelerate osteoblast cell growth. Characteristic examinations of the scaffolds were performed by micrometer, SEM, contact angle measurement system, ATR-FTIR, tensile machine and swelling experiments. The thickness of all electrospun scaffolds was determined in the range of 0.010±0.001-0.012±0.002 mm. In order to optimize electrospinning processes, suitable bead-free and uniform scaffolds were selected by using SEM images. Blending of PCL with chitosan resulted in better hydrophilicity for the PCL/chitosan scaffolds. The characteristic peaks of PCL and chitosan in the blend and layer by layer nanofibers were observed. The PCL/chitosan/PCL layer by layer structure had higher elastic modulus and tensile strength values than both individual PCL and chitosan structures. The layer by layer scaffolds exhibited the PBS absorption values of 184.2; 197.2% which were higher than those of PCL scaffolds but lower than those of PCL/chitosan blend scaffolds. SaOs-2 osteosarcoma cell culture studies showed that the highest ALP activities belonged to novel PCL/chitosan/PCL layer by layer scaffolds meaning better cell differentiation on the surfaces.
在这项研究中，天然可生物降解多糖壳聚糖和合成可生物降解聚合物聚（ε-己内酯）（PCL）被用于通过静电纺丝技术制备 3D 混合聚合物组织支架（PCL/壳聚糖共混物和 PCL/壳聚糖/PCL 层层支架）。通过添加 HA 来加速成骨细胞生长，开发了这些混合支架。通过微尺度、SEM、接触角测量系统、ATR-FTIR、拉伸机和溶胀实验对支架进行了特征检测。所有静电纺丝支架的厚度均在 0.010±0.001-0.012±0.002mm 的范围内确定。为了优化静电纺丝工艺，通过 SEM 图像选择了合适的无珠且均匀的支架。PCL 与壳聚糖共混后，PCL/壳聚糖支架的亲水性更好。在共混和层层纳米纤维中观察到了 PCL 和壳聚糖的特征峰。PCL/壳聚糖/PCL 层层结构的弹性模量和拉伸强度值均高于单独的 PCL 和壳聚糖结构。层层支架的 PBS 吸收值为 184.2%；197.2%高于 PCL 支架，但低于 PCL/壳聚糖共混支架。SaOs-2 骨肉瘤细胞培养研究表明，新型 PCL/壳聚糖/PCL 层层支架的碱性磷酸酶活性最高，意味着在表面上具有更好的细胞分化。
