耐砷细菌在生长培养基中溶解砷，并增加砷超富集植物蜈蚣草的生长。
Arsenic-resistant bacteria solubilized arsenic in the growth media and increased growth of arsenic hyperaccumulator Pteris vittata L.
机构信息
Soil and Water Science Department, University of Florida, Gainesville, FL 32611, USA.
出版信息
Bioresour Technol. 2011 Oct;102(19):8756-61. doi: 10.1016/j.biortech.2011.07.064. Epub 2011 Jul 24.
The role of arsenic-resistant bacteria (ARB) in arsenic solubilization from growth media and growth enhancement of arsenic-hyperaccumulator Pteris vittata L. was examined. Seven ARB (tolerant to 10 mM arsenate) were isolated from the P. vittata rhizosphere and identified by 16S rRNA sequencing as Pseudomonas sp., Comamonas sp. and Stenotrophomonas sp. During 7-d hydroponic experiments, these bacteria effectively solubilized arsenic from the growth media spiked with insoluble FeAsO₄ and AlAsO₄ minerals (from < 5 μg L⁻¹ to 5.04-7.37 mg L⁻¹ As) and enhanced plant arsenic uptake (from 18.1-21.9 to 35.3-236 mg kg⁻¹ As in the fronds). Production of (1) pyochelin-type siderophores by ARB (fluorescent under ultraviolet illumination and characterized with thin layer chromatography) and (2) root exudate (dissolved organic C) by P. vittata may be responsible for As solubilization. Increase in P. vittata root biomass from 1.5-2.2 to 3.4-4.2 g/plant dw by ARB and by arsenic was associated with arsenic-induced plant P uptake. Arsenic resistant bacteria may have potential to enhance phytoremediation of arsenic-contaminated soils by P. vittata.
砷抗性细菌 (ARB) 在从生长介质中溶解砷和促进砷超积累植物蜈蚣草 (Pteris vittata L.) 生长方面的作用进行了研究。从蜈蚣草根际分离到 7 株 ARB（耐受 10 mM 砷酸盐），通过 16S rRNA 测序鉴定为假单胞菌、丛毛单胞菌和寡养单胞菌。在 7 天的水培实验中，这些细菌有效地从生长介质中溶解了添加的难溶性 FeAsO₄ 和 AlAsO₄ 矿物中的砷（从 < 5 μg L⁻¹ 增加到 5.04-7.37 mg L⁻¹ As），并增强了植物对砷的吸收（从 18.1-21.9 增加到 35.3-236 mg kg⁻¹ As 在叶子中）。ARB 产生的（1）吡咯烷酮型铁载体（在紫外线照射下发出荧光，并通过薄层层析进行鉴定）和（2）蜈蚣草根际分泌物（溶解有机 C）可能是砷溶解的原因。ARB 和砷使蜈蚣草根生物量从 1.5-2.2 增加到 3.4-4.2 g/plant dw，与砷诱导的植物 P 吸收增加有关。砷抗性细菌可能有潜力通过蜈蚣草增强受砷污染土壤的植物修复。
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