Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Yamada-Oka, Suita, Japan.
J Biochem. 2011 Nov;150(5):563-8. doi: 10.1093/jb/mvr096. Epub 2011 Aug 12.
Old yellow enzyme (OYE) is an NADPH oxidoreductase capable of reducing a variety of compounds. It contains flavin mononucleotide (FMN) as a prosthetic group. A ternary complex structure of OYE from Trypanosoma cruzi (TcOYE) with FMN and one of the substrates, p-hydroxybenzaldehyde, shows a striking movement around the active site upon binding of the substrate. From a structural comparison of other OYE complexed with 12-oxophytodienoate, we have constructed a complex structure with another substrate, prostaglandin H(2) (PGH(2)), to provide a proposed stereoselective reaction mechanism for the reduction of PGH(2) to prostaglandin F(2α) by TcOYE.
老黄酶（OYE）是一种 NADPH 氧化还原酶，能够还原多种化合物。它含有黄素单核苷酸（FMN）作为辅基。来自克氏锥虫（TcOYE）的 OYE 与 FMN 和一种底物对羟基苯甲醛的三元复合物结构显示，在结合底物时，活性位点周围会发生显著的运动。通过与其他 OYE 与 12-氧代植二烯酸复合物的结构比较，我们构建了与另一种底物前列腺素 H2（PGH2）的复合物结构，为 TcOYE 将 PGH2 还原为前列腺素 F2α 的立体选择性反应机制提供了一个建议。
