Palo Alto Research Center, 3333 Coyote Hill Road, Palo Alto, CA 94304, USA.
Lab Chip. 2011 Oct 7;11(19):3313-9. doi: 10.1039/c1lc20354a. Epub 2011 Aug 15.
We demonstrate rapid mixing of sub-microlitre droplets (250 nl) using miniaturized magnetic stir bars (400 μm × 200 μm × 15 μm). The stir bars are fabricated using laser micromachining and placed on the substrate on which the drops are manipulated. They are activated by an externally applied magnetic field and used in combination with on-demand drop merging in enthalpy arrays. This technique results in a 10-fold increase in mixing rate, and a mixing time constant of about 2 s. Drop mixing times are measured by Förster resonance energy transfer (FRET) and verified by thermodynamic measurements of binding and enzymatic reactions.
我们使用微型化的磁性搅拌棒(400μm×200μm×15μm)展示了亚微升(250nl)液滴的快速混合。搅拌棒采用激光微加工制造,并放置在操控液滴的基底上。它们在外加磁场的作用下被激活,并与按需合并的滴在焓数组中结合使用。这种技术使混合速率提高了 10 倍,混合时间常数约为 2 秒。通过Förster 共振能量转移(FRET)测量混合时间,并通过结合和酶反应的热力学测量进行验证。