CD8+ 细胞毒性 T 淋巴细胞中细胞裂解颗粒胞吐作用的成像揭示了一种完全融合的改良形式。
Imaging of lytic granule exocytosis in CD8+ cytotoxic T lymphocytes reveals a modified form of full fusion.
机构信息
Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Heath, Bethesda, MD 20892-8017, USA.
出版信息
Cell Immunol. 2011;271(2):267-79. doi: 10.1016/j.cellimm.2011.07.004. Epub 2011 Jul 24.
Here we imaged the exocytosis of lytic granules from human CD8(+) cytotoxic T lymphocytes using rapid total internal reflection microscopy, Lamp-1 tagged with mGFP to follow the fate of the lytic granule membrane, and granzyme A, granzyme B or serglycin tagged with mRFP to follow the fate of lytic granule cargo. Lytic granules were released by full fusion with the plasma membrane, such that the entire granule content for all three cargos visualized was released on a subsecond time scale. The behavior of GFP-Lamp-1 was, however, more complex. While it entered the plasma membrane in all cases, the extent to which it then diffused away from the site of exocytosis varied from nearly complete to highly restricted. Finally, the diffusion properties upon release of the three cargos examined put an upper limit on the size of the macromolecular complex of granzyme and serglycin that is presented to the target cell.
在这里，我们使用快速全内反射显微镜观察了人 CD8(+)细胞毒性 T 淋巴细胞中溶酶体的胞吐作用，使用 Lamp-1 标记的 mGFP 来跟踪溶酶体膜的命运，用 granzymes A、B 或 serglycin 标记的 mRFP 来跟踪溶酶体 cargo 的命运。溶酶体通过与质膜完全融合而释放，使得所有三种 cargo 的全部内容在亚秒时间尺度上释放。然而，GFP-Lamp-1 的行为更为复杂。尽管在所有情况下它都进入了质膜，但随后它从胞吐部位扩散的程度从几乎完全扩散到高度受限不等。最后，所研究的三种 cargo 的释放扩散特性对递呈给靶细胞的 granzyme 和 serglycin 大分子复合物的大小施加了上限。
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