State Key Laboratory of Agrobiotechnology, Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Poult Sci. 2011 Sep;90(9):1941-7. doi: 10.3382/ps.2011-01489.
Toll-like receptors (TLR) mediate immune responses via recognition of pathogen-associated molecular patterns, thus playing important roles in the host defense. Polymorphisms of TLR may affect their recognition of pathogen-associated molecular patterns, leading to varied host resistance to pathogenic infections. However, little is known regarding the polymorphisms of chicken TLR (ChTLR) among breeds. In this study, we cloned ChTLR1 type 1 and type 2 genes from 9 chicken breeds (2 commercial breeds and 7 Chinese native breeds) and analyzed their sequences. We found 27 amino acid polymorphism sites in ChTLR: 14 sites in type 1 (8 sites in the extracellular domain, 5 sites in the cytoplasmic domain, and 1 site in the transmembrane domain) and 13 sites in type 2 (11 sites in the extracellular domain, 1 site in the cytoplasmic domain, and 1 site in the transmembrane domain). These results demonstrate that ChTLR1 type 1 and type 2 genes are polymorphic among chicken breeds, suggesting a varied resistance among breeds of chicken. This information provides new consideration for future poultry breeding and breed improvement.
Toll 样受体 (TLR) 通过识别病原体相关分子模式来介导免疫反应，从而在宿主防御中发挥重要作用。TLR 的多态性可能影响其对病原体相关分子模式的识别，导致宿主对致病感染的抵抗力不同。然而，关于鸡 TLR (ChTLR) 在品种间的多态性知之甚少。本研究从 9 个鸡品种（2 个商业品种和 7 个中国本地品种）中克隆了 ChTLR1 1 型和 2 型基因，并分析了它们的序列。我们在 ChTLR 中发现了 27 个氨基酸多态性位点：1 型中的 14 个位点（胞外域中的 8 个位点、胞质域中的 5 个位点和跨膜域中的 1 个位点）和 2 型中的 13 个位点（胞外域中的 11 个位点、胞质域中的 1 个位点和跨膜域中的 1 个位点）。这些结果表明，ChTLR1 1 型和 2 型基因在鸡品种间是多态的，表明鸡品种间存在不同的抵抗力。这些信息为未来家禽的选育和品种改良提供了新的考虑。
