Burkness Eric C, Dively Galen, Patton Terry, Morey Amy C, Hutchison William D
University of Minnesota, Department of Entomology, St. Paul, MN, USA.
GM Crops. 2010 Nov-Dec;1(5):337-43. doi: 10.4161/gmcr.1.5.14765.
Sweet corn, Zea mays L., transformed to express a novel vegetative insecticidal protein, Vip3A (event MIR162, Syngenta Seeds, Inc..), produced by the bacterium, Bacillus thuringiensis (Bt), was evaluated over four field seasons in Maryland and two field seasons in Minnesota for efficacy against the corn earworm, Helicoverpa zea (Boddie). Hybrids expressing the Vip3A protein and pyramided in hybrids also expressing the Cry1Ab Bt protein (event Bt11, ATTRIBUTE(®), Syngenta Seeds, Inc.) were compared to hybrids expressing only Cry1Ab or to genetically similar non-Bt hybrids each year. In addition to H. zea efficacy, results for Ostrinia nubilalis (Hübner) and Spodoptera frugiperda (J.E. Smith) are presented. Over all years and locations, the non-Bt hybrids, without insecticide protection, averaged between 43 and 100% ears infested with a range of 0.24 to 1.74 H. zea larvae per ear. By comparison, in the pyramided Vip3A x Cry1Ab hybrids, no larvae were found and only minimal kernel damage (likely due to other insect pests) was recorded. Hybrids expressing only Cry1Ab incurred a moderate level of H. zea feeding damage, with surviving larvae mostly limited to the first or second instar as a result of previously documented growth inhibition from Cry1Ab. These results suggest that the Vip3A protein, pyramided with Cry1Ab, appears to provide the first "high-dose" under field conditions and will be valuable for ongoing resistance management.
甜玉米，玉米（Zea mays L.），经转化后表达一种新型营养期杀虫蛋白Vip3A（事件MIR162，先正达种子公司），该蛋白由苏云金芽孢杆菌（Bt）产生。在马里兰州进行了四个田间季节以及在明尼苏达州进行了两个田间季节的评估，以测定其对玉米穗虫（Helicoverpa zea (Boddie)）的防治效果。每年将表达Vip3A蛋白且与也表达Cry1Ab Bt蛋白（事件Bt11，ATTRIBUTE(®)，先正达种子公司）的杂交种进行聚合的杂交种，与仅表达Cry1Ab的杂交种或遗传上相似的非Bt杂交种进行比较。除了对玉米穗虫的防治效果外，还给出了欧洲玉米螟（Ostrinia nubilalis (Hübner)）和草地贪夜蛾（Spodoptera frugiperda (J.E. Smith)）的防治结果。在所有年份和地点，没有杀虫剂保护的非Bt杂交种，平均有43%至100%的果穗受到侵染，每穗有0.24至1.74头玉米穗虫幼虫。相比之下，在Vip3A与Cry1Ab聚合的杂交种中，未发现幼虫，仅记录到极轻微的籽粒损伤（可能是由于其他害虫造成的）。仅表达Cry1Ab的杂交种遭受了中等程度的玉米穗虫取食损伤，由于先前记录的Cry1Ab对其生长的抑制作用，存活的幼虫大多限于一龄或二龄。这些结果表明，与Cry1Ab聚合的Vip3A蛋白似乎在田间条件下提供了首个“高剂量”，对于正在进行的抗性管理将具有重要价值。
