Ilardi Vincenza, Nicola-Negri Elisa Di
CRA-Centro di Ricerca per la Patologia Vegetale, Rome, Italy.
GM Crops. 2011 Jan-Mar;2(1):24-33. doi: 10.4161/gmcr.2.1.15096.
Plum pox virus (PPV), a Potyvirus, is the causal agent of sharka, the most detrimental viral disease affecting stone fruit trees. This review focuses on research carried out to obtain PPV- resistant transgenic plants and on how biotechnological strategies evolved in light of the scientific advances made during the last several years. Successful RNA silencing strategies that confer high level of resistance to strains of PPV have been developed and tested under laboratory and greenhouse conditions. Moreover, field tests showed that transgene-mediated RNA silencing was effective in protecting plum plants against aphid-mediated PPV infection. The new emerging biotechnological approaches for conferring PPV resistance are discussed.
李痘病毒（PPV）属于马铃薯Y病毒属，是沙卡病的病原体，沙卡病是影响核果类果树最具危害性的病毒性疾病。本综述着重介绍了为获得抗PPV转基因植物所开展的研究，以及生物技术策略如何根据过去几年取得的科学进展而演变。已经开发出成功的RNA沉默策略，可在实验室和温室条件下对PPV毒株产生高水平抗性并进行了测试。此外，田间试验表明，转基因介导的RNA沉默可有效保护李树植株免受蚜虫介导的PPV感染。本文还讨论了赋予PPV抗性的新兴生物技术方法。
