Shofian Norshahida Mohamad, Hamid Azizah Abdul, Osman Azizah, Saari Nazamid, Anwar Farooq, Dek Mohd Sabri Pak, Hairuddin Muhammad Redzuan
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia; E-Mails:
Int J Mol Sci. 2011;12(7):4678-92. doi: 10.3390/ijms12074678. Epub 2011 Jul 20.
The effects of freeze-drying on antioxidant compounds and antioxidant activity of five tropical fruits, namely starfruit (Averrhoa carambola L.), mango (Mangifera indica L.), papaya (Carica papaya L.), muskmelon (Cucumis melo L.), and watermelon Citruluss lanatus (Thunb.) were investigated. Significant (p < 0.05) differences, for the amounts of total phenolic compounds (TPC), were found between the fresh and freeze-dried fruit samples, except muskmelon. There was no significant (p > 0.05) change, however, observed in the ascorbic acid content of the fresh and freeze-dried fruits. Similarly, freeze-drying did not exert any considerable effect on β-carotene concentration of fruits, except for mango and watermelon, where significantly (p < 0.05) higher levels were detected in the fresh samples. The results of DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging and reducing power assays revealed that fresh samples of starfruit and mango had relatively higher antioxidant activity. In case of linoleic acid peroxidation inhibition measurement, a significant (p < 0.05) but random variation was recorded between the fresh and freeze-dried fruits. Overall, in comparison to β-carotene and ascorbic acid, a good correlation was established between the result of TPC and antioxidant assays, indicating that phenolics might have been the dominant compounds contributing towards the antioxidant activity of the fruits tested.
研究了冷冻干燥对五种热带水果,即杨桃(Averrhoa carambola L.)、芒果(Mangifera indica L.)、木瓜(Carica papaya L.)、甜瓜(Cucumis melo L.)和西瓜(Citruluss lanatus (Thunb.))抗氧化化合物及抗氧化活性的影响。除甜瓜外,新鲜水果样品和冷冻干燥水果样品之间的总酚化合物(TPC)含量存在显著(p < 0.05)差异。然而,新鲜水果和冷冻干燥水果的抗坏血酸含量没有显著(p > 0.05)变化。同样,冷冻干燥对水果的β-胡萝卜素浓度没有产生任何显著影响,除了芒果和西瓜,新鲜样品中的β-胡萝卜素浓度显著(p < 0.05)更高。二苯基苦味酰基自由基(DPPH)清除和还原能力测定结果表明,杨桃和芒果的新鲜样品具有相对较高的抗氧化活性。在亚油酸过氧化抑制测定中,新鲜水果和冷冻干燥水果之间记录到显著(p < 0.05)但随机的变化。总体而言,与β-胡萝卜素和抗坏血酸相比,TPC结果与抗氧化测定之间建立了良好的相关性,表明酚类物质可能是所测试水果抗氧化活性的主要贡献化合物。