Cao Yanping, Shan Anshan, Ma Qingquan, Yin Jiajia
Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China.
Sheng Wu Gong Cheng Xue Bao. 2011 May;27(5):684-9.
The technology of genetic engineering has been widely used to express macromolecules such as enzymes. However, it is difficult to detect and purify the micromolecules such as small peptides, because of their instability and degradability. Construction of multi-copy recombinant expression plasmid can be achieved by inserting multiple target genes or expression cassette containing target genes with the same orientation into expression vector. This is effective to increase the expression level of small peptides. In this article we described four methods in order to provide some optional methods and ideas for the expression of active small peptides.
基因工程技术已被广泛用于表达诸如酶等大分子。然而，由于小肽等小分子的不稳定性和可降解性，难以对其进行检测和纯化。通过将多个目标基因或包含同向目标基因的表达盒插入表达载体中，可以构建多拷贝重组表达质粒。这对于提高小肽的表达水平是有效的。在本文中，我们描述了四种方法，以便为活性小肽的表达提供一些可供选择的方法和思路。
